Statistical analysis was performed using a Fisher’s exact t-test comparing embryos treated with the MT ATG or the Lss inhibitor to embryos injected with standard control morpholino. Std, standard control morpholino; MT ATG, etv7 translational-inhibiting morpholino; Lss inhibitor, embryos treated with 50 nM Lss inhibitor.

Analysis was performed using Fisher’s exact t-test comparing MT ATG with cholesterol or inhibitor with cholesterol to Std with cholesterol. NS, not significant and determines the statistical difference between the Std and the Std with cholesterol.