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Table 1.

Energy-transfer efficiency values (E) between donor-acceptor pairs before and after LPS stimulation

Donor (FITC)Acceptor (Rhodamine)E±▵E (%)
Before LPS stimulation   
CD14 CD14 39±1.0 
CD14 GM-1 ganglioside 32±1.2 
Hsp70 GM-1 ganglioside 17±1.2 
Hsp90 GM-1 ganglioside 13±1.0 
TLR4 GM-1 ganglioside 6±0.8 
CXCR4 GM-1 ganglioside 7±2.0 
GDF5 GM-1 ganglioside 3±1.0 
After LPS stimulation   
CD14 GM-1 ganglioside 32±1.8 
Hsp70 GM-1 ganglioside 31±2.0 
Hsp90 GM-1 ganglioside 28±0.5 
TLR4 GM-1 ganglioside 34±0.8 
CXCR4 GM-1 ganglioside 26±1.0 
GDF5 GM-1 ganglioside 19±1.2 
Donor (FITC)Acceptor (Rhodamine)E±▵E (%)
Before LPS stimulation   
CD14 CD14 39±1.0 
CD14 GM-1 ganglioside 32±1.2 
Hsp70 GM-1 ganglioside 17±1.2 
Hsp90 GM-1 ganglioside 13±1.0 
TLR4 GM-1 ganglioside 6±0.8 
CXCR4 GM-1 ganglioside 7±2.0 
GDF5 GM-1 ganglioside 3±1.0 
After LPS stimulation   
CD14 GM-1 ganglioside 32±1.8 
Hsp70 GM-1 ganglioside 31±2.0 
Hsp90 GM-1 ganglioside 28±0.5 
TLR4 GM-1 ganglioside 34±0.8 
CXCR4 GM-1 ganglioside 26±1.0 
GDF5 GM-1 ganglioside 19±1.2 

Energy transfer between different pairs was detected before and after LPS stimulation from the increase in donor fluorescence after acceptor photobleaching. Data are means±s.d. values from several independent experiments.

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