Detection of the various VSG forms
| VSG form . | Anti-MITat 1.2 Ab . | Anti-CRD Ab . | Streptavidin-HRP . |
|---|---|---|---|
| Mature VSG | + | (+)* | - |
| Pro VSG | + | - | - |
| Biotinylated VSG | + | - | + |
| VSG form . | Anti-MITat 1.2 Ab . | Anti-CRD Ab . | Streptavidin-HRP . |
|---|---|---|---|
| Mature VSG | + | (+)* | - |
| Pro VSG | + | - | - |
| Biotinylated VSG | + | - | + |
Anti CRD antibodies recognize soluble (s) but not membrane-form (mf) VSG. sVSG is formed from mfVSG during lysis by action of the endogenous GPI specific phospholipase C.