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Table 1.

Plasmids used in the study for expression of UL14 mutant proteins

Plasmid (pcDNA3-)UL14 mutant protein (amino acids)
UL14 Full-length HSV-2 UL14 (1-219) 
UL14ND20 N-terminus shortened UL14 (21-219) 
UL14ND40 N-terminus shortened UL14 (41-219) 
UL14ND60 N-terminus shortened UL14 (61-219) 
UL14ND80 N-terminus shortened UL14 (81-219) 
UL14ND120 N-terminus shortened UL14 (121-219) 
UL14CD40 C-terminus shortened UL14 (1-189) 
UL14K51A Lys51 replaced with Ala 
UL14R60A Arg60 replaced with Ala 
UL14R(60,64)A Arg60 and -64 replaced with Ala 
PM3 Lys51, Arg60 and -64 replaced with Ala 
UL14D(51-90) 40 residues (51-90) replaced with Asp-Ile 
UL14D(121-180) 60 residues (121-180) replaced with Asp-Ile 
Plasmid (pcDNA3-)UL14 mutant protein (amino acids)
UL14 Full-length HSV-2 UL14 (1-219) 
UL14ND20 N-terminus shortened UL14 (21-219) 
UL14ND40 N-terminus shortened UL14 (41-219) 
UL14ND60 N-terminus shortened UL14 (61-219) 
UL14ND80 N-terminus shortened UL14 (81-219) 
UL14ND120 N-terminus shortened UL14 (121-219) 
UL14CD40 C-terminus shortened UL14 (1-189) 
UL14K51A Lys51 replaced with Ala 
UL14R60A Arg60 replaced with Ala 
UL14R(60,64)A Arg60 and -64 replaced with Ala 
PM3 Lys51, Arg60 and -64 replaced with Ala 
UL14D(51-90) 40 residues (51-90) replaced with Asp-Ile 
UL14D(121-180) 60 residues (121-180) replaced with Asp-Ile 
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