FRET efficiency
| . | FRET efficiency (%)* . | . | |
|---|---|---|---|
| . | Interphase NE . | Telophase core region . | |
| mCFP-BAF/mVenus-BAF | 12.3±8.7 (n=7) | 26.7±14.2 (n=15) | |
| mCFP-BAF/mVenus-emerin | 15.2±10.6 (n=11) | 14.6±13.2 (n=29) | |
| mCFP-BAF/mVenus-emerin m24 | ND | 2.7±1.35 (n=10) | |
| mCFP-BAF/mVenus-Lamin A | 3.1±8.2 (n=10) | 23.0±12.0 (n=31) | |
| . | FRET efficiency (%)* . | . | |
|---|---|---|---|
| . | Interphase NE . | Telophase core region . | |
| mCFP-BAF/mVenus-BAF | 12.3±8.7 (n=7) | 26.7±14.2 (n=15) | |
| mCFP-BAF/mVenus-emerin | 15.2±10.6 (n=11) | 14.6±13.2 (n=29) | |
| mCFP-BAF/mVenus-emerin m24 | ND | 2.7±1.35 (n=10) | |
| mCFP-BAF/mVenus-Lamin A | 3.1±8.2 (n=10) | 23.0±12.0 (n=31) | |
FRET efficiency (%) = [1–(donor intensity before acceptor photobleaching/donor intensity after acceptor photobleaching)] × 100