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Table 1.

FRET efficiency

FRET efficiency (%)*
Interphase NE Telophase core region
mCFP-BAF/mVenus-BAF   12.3±8.7 (n=7)   26.7±14.2 (n=15)  
mCFP-BAF/mVenus-emerin   15.2±10.6 (n=11)   14.6±13.2 (n=29)  
mCFP-BAF/mVenus-emerin m24   ND   2.7±1.35 (n=10)  
mCFP-BAF/mVenus-Lamin A   3.1±8.2 (n=10)   23.0±12.0 (n=31)  
FRET efficiency (%)*
Interphase NE Telophase core region
mCFP-BAF/mVenus-BAF   12.3±8.7 (n=7)   26.7±14.2 (n=15)  
mCFP-BAF/mVenus-emerin   15.2±10.6 (n=11)   14.6±13.2 (n=29)  
mCFP-BAF/mVenus-emerin m24   ND   2.7±1.35 (n=10)  
mCFP-BAF/mVenus-Lamin A   3.1±8.2 (n=10)   23.0±12.0 (n=31)  
*

FRET efficiency (%) = [1–(donor intensity before acceptor photobleaching/donor intensity after acceptor photobleaching)] × 100

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