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Table 2.

Microtubule dynamics following osmotic stress

Strain Time before resumption of dynamic behavior following osmotic stress (minutes)
Wild type   37.8±1.2  
pmk1.Δ  38.5±0.9  
ssp1.Δ  37.3±3.5  
sty1.Δ  71.3±8.3* 
wis1.DD  24.5±1.7  
atf1.Δ  69.5±7.1  
srk1.Δ  39.0±1.9  
Strain Time before resumption of dynamic behavior following osmotic stress (minutes)
Wild type   37.8±1.2  
pmk1.Δ  38.5±0.9  
ssp1.Δ  37.3±3.5  
sty1.Δ  71.3±8.3* 
wis1.DD  24.5±1.7  
atf1.Δ  69.5±7.1  
srk1.Δ  39.0±1.9  

n=8 for all strains examined

*

The resumption of microtubule dynamics in sty1.Δ cultures was highly heterogenous, with 72 out of 400 cells failing to recover dynamics up to 2.5 hours after osmotic stress. The calculations of recovery time in sty1.Δ cultures therefore represent the time at which the first cell in the field of view exhibited a return to dynamic behaviour

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