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Table 2.

Water content in organs and carcasses of Rana sylvaticaadministered saline or saline containing urea, and sampled before or after freezing at –4°C

Saline treatedUrea loaded
Unfrozen   
   Heart 82.8±0.5 82.2±0.6 
   Liver 71.2±0.7 71.7±1.0 
   Muscle 79.2±0.2 78.5±0.4 
   Carcass 78.2±0.3 78.9±0.5 
Frozen/thawed   
   Heart 83.9±0.3 83.4±0.7 
   Liver 78.8±0.4* 78.3±0.5* 
   Muscle 79.2±0.4 78.3±0.5 
   Carcass 79.4±0.2 78.2±0.5 
Saline treatedUrea loaded
Unfrozen   
   Heart 82.8±0.5 82.2±0.6 
   Liver 71.2±0.7 71.7±1.0 
   Muscle 79.2±0.2 78.5±0.4 
   Carcass 78.2±0.3 78.9±0.5 
Frozen/thawed   
   Heart 83.9±0.3 83.4±0.7 
   Liver 78.8±0.4* 78.3±0.5* 
   Muscle 79.2±0.4 78.3±0.5 
   Carcass 79.4±0.2 78.2±0.5 

Water content is given as % fresh tissue mass. Values are means ±s.e.m.; N=5 frogs per group

Asterisk denotes that the mean for the frozen/thawed group differed significantly (P<0.05) from its unfrozen counterpart

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