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Table 1.

Concentrations of urea and glucose in plasma and organs of Rana sylvatica administered saline or saline containing urea, and sampled before or after freezing at –4°C

Urea
Glucose
Saline treatedUrea loadedSaline treatedUrea loaded
Unfrozen     
   Plasma 9.8±0.8 69.6±2.5* 1.9±0.4 2.4±0.2 
   Heart 14.6±1.5 80.6±5.3* 1.7±0.4 1.7±0.3 
   Liver 15.7±2.8 72.1±5.3* 3.9±0.4 4.1±0.3 
   Muscle 12.1±1.7 47.8±5.9* 1.0±0.2 1.3±0.1 
Frozen/thawed     
   Plasma 11.7±1.5 60.4±2.0* 28.1±2.0 42.3±3.8* 
   Heart 18.7±3.5 56.2±1.1* 18.6±2.1 53.7±3.9* 
   Liver 21.1±3.6 57.2±1.6* 19.9±2.1 69.9±10.9* 
   Muscle 19.5±3.3 56.1±2.2* 18.1±2.6 40.9±2.9* 
Urea
Glucose
Saline treatedUrea loadedSaline treatedUrea loaded
Unfrozen     
   Plasma 9.8±0.8 69.6±2.5* 1.9±0.4 2.4±0.2 
   Heart 14.6±1.5 80.6±5.3* 1.7±0.4 1.7±0.3 
   Liver 15.7±2.8 72.1±5.3* 3.9±0.4 4.1±0.3 
   Muscle 12.1±1.7 47.8±5.9* 1.0±0.2 1.3±0.1 
Frozen/thawed     
   Plasma 11.7±1.5 60.4±2.0* 28.1±2.0 42.3±3.8* 
   Heart 18.7±3.5 56.2±1.1* 18.6±2.1 53.7±3.9* 
   Liver 21.1±3.6 57.2±1.6* 19.9±2.1 69.9±10.9* 
   Muscle 19.5±3.3 56.1±2.2* 18.1±2.6 40.9±2.9* 

Concentrations of urea and glucose in plasma are μmol ml-1and in organs are μmol g–1 fresh tissue. Values are means± s.e.m.; N=5 frogs per group

Asterisk denotes that the mean for the urea-loaded group differed significantly (P<0.05) from its saline-treated counterpart

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