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Table 5.

qRT-PCR validation of candidate Hr39-regulated genes from microarray

takeout
Cyp4d21
AttC
Pbprp1
Hr39
AlleleMicro*qRT-PCRMicro*qRT-PCRMicro*qRT-PCRMicro*qRT-PCRMicro*qRT-PCR
ly92  62↑  12↑  2↓  90↓  20↓ 
03508  24↑  38↑    198↓  143↓ 
04443 84↑ 131↑ 27↑ 40↑ 58↓ 53↓ 45↓ 61↓ 2↓ 18↓ 
07154  573↑  184↑  2↓  165↓  393↓ 
takeout
Cyp4d21
AttC
Pbprp1
Hr39
AlleleMicro*qRT-PCRMicro*qRT-PCRMicro*qRT-PCRMicro*qRT-PCRMicro*qRT-PCR
ly92  62↑  12↑  2↓  90↓  20↓ 
03508  24↑  38↑    198↓  143↓ 
04443 84↑ 131↑ 27↑ 40↑ 58↓ 53↓ 45↓ 61↓ 2↓ 18↓ 
07154  573↑  184↑  2↓  165↓  393↓ 
*

Average fold change detected by microarray between wild type and Hr3904443 in reproductive tract.

Fold change detected by quantitative RT-PCR between wild type and the indicated Hr39 mutant allele in reproductive tract.

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