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Table 2.

Targeted overexpression of Dally and its effect on GSC number

Dissection time after eclosion
Day 5
Day 15
Genotype<7 spherical fusomes≥7 spherical fusomes<7 spherical fusomes≥7 spherical fusomes
C587GA14> UAS-dally 141 (73%)  53 (27%)  231 (100%) 
  n=194   n=231  
C587GAL4>UAS-TM-dally 104 (88%)  14 (12%) 46 (75%)  15 (25%) 
  n=118   n=61  
C587GAL4>UAS-sec-dally 196 (100%)  285 (100%)  
  n=196   n=285  
ptcGAL4>UAS-dally 54 (57%)  40 (43%) 9 (29%)  22 (71%) 
  n=94   n=31  
enGAL4>UAS-dally 203 (100%)  115 (100%)  
  n=203   n=115  
bab1-GAL4>UAS-dally 196 (99%)  2 (1%) 191 (85%)  34 (15%) 
  n=198   n=225  
nos-GAL4VP16>UASP-dally 58 (48%)  63 (52%) 12 (16%)  62 (84%) 
  n=121   n=74  
Dissection time after eclosion
Day 5
Day 15
Genotype<7 spherical fusomes≥7 spherical fusomes<7 spherical fusomes≥7 spherical fusomes
C587GA14> UAS-dally 141 (73%)  53 (27%)  231 (100%) 
  n=194   n=231  
C587GAL4>UAS-TM-dally 104 (88%)  14 (12%) 46 (75%)  15 (25%) 
  n=118   n=61  
C587GAL4>UAS-sec-dally 196 (100%)  285 (100%)  
  n=196   n=285  
ptcGAL4>UAS-dally 54 (57%)  40 (43%) 9 (29%)  22 (71%) 
  n=94   n=31  
enGAL4>UAS-dally 203 (100%)  115 (100%)  
  n=203   n=115  
bab1-GAL4>UAS-dally 196 (99%)  2 (1%) 191 (85%)  34 (15%) 
  n=198   n=225  
nos-GAL4VP16>UASP-dally 58 (48%)  63 (52%) 12 (16%)  62 (84%) 
  n=121   n=74  

Flies were raised at 29°C. The number of spherical fusomes is equal to the number of GSC-like cells and cystoblasts. `7' is used because generally 6 is the maximal number of spherical fusomes observed in wild type (three in GSCs and three in cystoblasts). Numbers in the table are the counts of germaria and their percentage of the total (n) examined at the same stage of adulthood.

sec, secreted; TM, transmembrane.

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