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Table 1.

Scoring of GSC-like cells in dally mutants and Dally-RNAi flies

Dissection time after eclosion
Day 3
Day 10*
Genotype2-3 GSCs/niche1 GSC/niche0 GSCs/niche2-3 GSCs/niche1 GSC/niche0 GSCs/niche
w1118 126 (100%) 210 (98%) 2 (1%) 1 (1%) 
  n=126   n=213  
dally305 126 (77%) 20 (12%) 17 (11%) 37 (23%) 34 (21%) 91(56%) 
  n=163   n=162  
dallygem 47 (16%) 19 (7%) 222(77%) 25 (8%) 3 (1%) 288 (91%) 
  n=288   n=316  
dally80 14 (31%) 11 (24%) 20 (45%)    
  n=45  ND (rarely survive to day 10)   
dallyPZ/dally80 52 (84%) 1 (2%) 9 (14%) 16 (39%) 1 (2%) 24 (59%) 
  n=62   n=41  
dallygem/dallygem, UAS-dally 51 (20%) 10 (4%) 184 (76%) 35 (18%) 2 (1%) 147 (80%) 
  n=245   n=184  
en-GAL4; dallygem/dallygem, UAS-dally 107 (56%) 11 (6%) 73 (38%) 97 (48%) 5 (2%) 99 (50%) 
  n=191   n=201  
bab1-GAL4, dallygem/dallygem, UAS-dally 248 (80%) 14 (4%) 51 (16%) 220 (74%) 11 (4%) 65 (22%) 
  n=313   n=296  
C587-GAL4;;dallygem/dallygem, UAS-dally 251(81%) 2 (1%) 57 (18%) 119(78%) 3 (2%) 30 (20%) 
  n=310   n=152  
dallygem, DadP1883 66 (66%) 14 (14%) 21 (20%) 17 (26%) 3 (5%) 44 (69%) 
  n=101   n=64 (day 6)  
bab1-GAL4>UAS-dallyRNAi 85 (94%) 2 (2%) 3 (4%) 79 (65%) 7 (6%) 36 (29%) 
  n=90   n=122 (day 10-12)  
bab1-GAL4>UAS-sec-dally    98 (84%) 17 (14%) 2 (2%) 
  ND   n=117 (day 10-13)  
Dissection time after eclosion
Day 3
Day 10*
Genotype2-3 GSCs/niche1 GSC/niche0 GSCs/niche2-3 GSCs/niche1 GSC/niche0 GSCs/niche
w1118 126 (100%) 210 (98%) 2 (1%) 1 (1%) 
  n=126   n=213  
dally305 126 (77%) 20 (12%) 17 (11%) 37 (23%) 34 (21%) 91(56%) 
  n=163   n=162  
dallygem 47 (16%) 19 (7%) 222(77%) 25 (8%) 3 (1%) 288 (91%) 
  n=288   n=316  
dally80 14 (31%) 11 (24%) 20 (45%)    
  n=45  ND (rarely survive to day 10)   
dallyPZ/dally80 52 (84%) 1 (2%) 9 (14%) 16 (39%) 1 (2%) 24 (59%) 
  n=62   n=41  
dallygem/dallygem, UAS-dally 51 (20%) 10 (4%) 184 (76%) 35 (18%) 2 (1%) 147 (80%) 
  n=245   n=184  
en-GAL4; dallygem/dallygem, UAS-dally 107 (56%) 11 (6%) 73 (38%) 97 (48%) 5 (2%) 99 (50%) 
  n=191   n=201  
bab1-GAL4, dallygem/dallygem, UAS-dally 248 (80%) 14 (4%) 51 (16%) 220 (74%) 11 (4%) 65 (22%) 
  n=313   n=296  
C587-GAL4;;dallygem/dallygem, UAS-dally 251(81%) 2 (1%) 57 (18%) 119(78%) 3 (2%) 30 (20%) 
  n=310   n=152  
dallygem, DadP1883 66 (66%) 14 (14%) 21 (20%) 17 (26%) 3 (5%) 44 (69%) 
  n=101   n=64 (day 6)  
bab1-GAL4>UAS-dallyRNAi 85 (94%) 2 (2%) 3 (4%) 79 (65%) 7 (6%) 36 (29%) 
  n=90   n=122 (day 10-12)  
bab1-GAL4>UAS-sec-dally    98 (84%) 17 (14%) 2 (2%) 
  ND   n=117 (day 10-13)  

ND, not determined.

GSCs were identified by the morphology of fusome staining (αSpec or Hts). Numbers in the table are the counts of germarium and their percentage of the total (n) germaria examined at the same stage of adulthood. Flies of RNAi experiments were raised at 29°C.

*

Unless otherwise specified, adult day 10 samples were used.

About half of these ovarioles contained more than three GSCs.

Approximately 80% of these ovarioles contained more than three GSCs.

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