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Table 2.

Characteristics of the intestinal antibodies

Antibody nameAntigen size on western blot (kDa)Expression first seen at (days)Structures labelled
2F11 Four major bands at 170, 120, 90 and 35 Cytoplasm of enteroendocrine and goblet cells (but no labelling of the mucus compartment). The 3 markers label identical cell types as determined by double labelling 
4B7/2 35  
6G5 n.d.  
3G12 Three major bands at 175, 120 and 70 Mucus compartment and cytoplasm of goblet cells, in the posterior gut mainly 
4B7/1 n.d. Brush border of enterocytes (apical ends of microvilli) 
4E8 200  
2H9 170 Lateral membrane and basal region of epithelial cells 
5F11 n.d. Basement membrane 
Antibody nameAntigen size on western blot (kDa)Expression first seen at (days)Structures labelled
2F11 Four major bands at 170, 120, 90 and 35 Cytoplasm of enteroendocrine and goblet cells (but no labelling of the mucus compartment). The 3 markers label identical cell types as determined by double labelling 
4B7/2 35  
6G5 n.d.  
3G12 Three major bands at 175, 120 and 70 Mucus compartment and cytoplasm of goblet cells, in the posterior gut mainly 
4B7/1 n.d. Brush border of enterocytes (apical ends of microvilli) 
4E8 200  
2H9 170 Lateral membrane and basal region of epithelial cells 
5F11 n.d. Basement membrane 

n.d., not determined.

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