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Table 4.

Expression of Dlx genes in limbs of p63 mutant embryos

A
 

 

 

 

 

 

 

 
Target mRNA
 
Dlx1
 
Dlx2
 
Dlx5
 
Dlx6
 
Dlx6as
 
Dactylyn
 
Perp
 
FL*
 

 

 

 

 

 

 

 
+/+ 1 
p63−/− 0.6 0.5 0.5 0.3 0.5 1.2 0.2 
p63EEC/EEC
 
0.5
 
0.4
 
0.3
 
0.3
 
2
 
1.5
 
0.55
 
HL*
 

 

 

 

 

 

 

 
+/+ 
p63−/− 0.35 0.25 0.25 0.3 1.3 0.3 
p63EEC/EEC
 
0.25
 
0.25
 
0.2
 
0.2
 
3
 
1.3
 
0.4
 
B
 

 

 

 

 

 

 

 
Target mRNA
 
Dlx1
 
Dlx2
 
Dlx5
 
Dlx6
 
Perp
 

 

 
FL
 

 

 

 

 

 

 

 
+/+ 1   
p63+/− 1.2 1.6 1.5 1.3 1.0   
p63+/EEC
 
1.2
 
1.3
 
2
 
1.1
 
1.0
 

 

 
HL
 

 

 

 

 

 

 

 
+/+   
p63+/− 0.6 0.6 0.5 0.5 0.5   
p63+/EEC 0.9 0.9 0.6 0.55 0.7   
A
 

 

 

 

 

 

 

 
Target mRNA
 
Dlx1
 
Dlx2
 
Dlx5
 
Dlx6
 
Dlx6as
 
Dactylyn
 
Perp
 
FL*
 

 

 

 

 

 

 

 
+/+ 1 
p63−/− 0.6 0.5 0.5 0.3 0.5 1.2 0.2 
p63EEC/EEC
 
0.5
 
0.4
 
0.3
 
0.3
 
2
 
1.5
 
0.55
 
HL*
 

 

 

 

 

 

 

 
+/+ 
p63−/− 0.35 0.25 0.25 0.3 1.3 0.3 
p63EEC/EEC
 
0.25
 
0.25
 
0.2
 
0.2
 
3
 
1.3
 
0.4
 
B
 

 

 

 

 

 

 

 
Target mRNA
 
Dlx1
 
Dlx2
 
Dlx5
 
Dlx6
 
Perp
 

 

 
FL
 

 

 

 

 

 

 

 
+/+ 1   
p63+/− 1.2 1.6 1.5 1.3 1.0   
p63+/EEC
 
1.2
 
1.3
 
2
 
1.1
 
1.0
 

 

 
HL
 

 

 

 

 

 

 

 
+/+   
p63+/− 0.6 0.6 0.5 0.5 0.5   
p63+/EEC 0.9 0.9 0.6 0.55 0.7   
*

FL, forelimb; HL, hindlimb. The genotypes of p63(Brdm2 strain) and p63EEC (R279H) mutant limbs are indicated.

Values are normalized to the wild-type (+/+) sample, set=1. An external calibration sample was used, GADPH mRNA was determined as a positive control reference. Most determinations were repeated three times.

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