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Table 1.

Timing and sample size for measurements of energy and water budget components in reproductive (N=55) and non-reproductive(N=28) female mice exposed to 30°C

		Sample size^{^b}
Measurement	Days of reproduction^{^a}	Repr.	Non-repr.	Litters	Pups
DEE by DLW method	14	24 BC	17^{^c}	—	—
Water turnover by DLW method	14	24 BC^{^d}	24^{^d}	—	43 B
Total body water by desiccation^{^e}	14	8^{^f}	9^{^f}	—	46
Faecal and urinary water loss	14 and 15	5 C	5	—	—
Evaporative water loss^{^g}	15	5 C	5	—	—
RMR by respirometry	14	—	—	23 C	10 BC
Milk composition	15	12 C	—	—	—

		Sample size^{^b}
Measurement	Days of reproduction^{^a}	Repr.	Non-repr.	Litters	Pups
DEE by DLW method	14	24 BC	17^{^c}	—	—
Water turnover by DLW method	14	24 BC^{^d}	24^{^d}	—	43 B
Total body water by desiccation^{^e}	14	8^{^f}	9^{^f}	—	46
Faecal and urinary water loss	14 and 15	5 C	5	—	—
Evaporative water loss^{^g}	15	5 C	5	—	—
RMR by respirometry	14	—	—	23 C	10 BC
Milk composition	15	12 C	—	—	—

Non-reproductive females were measured on the same days as reproductive females.

DEE, daily energy expenditure; DLW, doubly labelled water; RMR, resting metabolic rate; Repr., reproductive females; Non-repr.,non-reproductive females.

Numbers indicate days after parturition (day 0 of lactation)

B and C indicate the same groups of reproductive females as in Król and Speakman (2003)

for 24 females injected with DLW, the blood samples from only 17 individuals were sufficiently large to run both ²H and ¹⁸O analyses

from ²H turnover during measurements of DEE

measured from separate groups of mice, from which no other measurements were taken

from Król and Speakman(1999)

measured in the same individuals as faecal and urinary water loss

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