The Company of Biologists

Table 2.

Responses of adult A. variegatum to entrained rumen odour and to individual chemostimulants of the rumen fluid odour and their mixtures

Treatment	Ticks tested (N)	Source dose	% Attraction^¹	% Change in speed^¹
Entrained rumen fluid odour	17	1/10 dilution	+42^{^***}^,a	+4^e,f
Single components
Butanoic acid	16	10 ng	+34^{^***}^,a,b	–34^{^**}^,f,g
Isobutanoic acid	29	10 ng–1 μg	+32^{^***}^,a,b	–9^{^*}^,e,f
4-Methylphenol	11	10 ng	+24^{^**}^,b,c	–2^e
3-Methylindole	20	10 ng	+12^{^*}^,b,c	–7^e,f
Acetic acid	14	1 μg	+15^{^*}^,b,c	+3^e
Propanoic acid	20	10 ng	+22^{^***}^,a,b	–12^{^***}^,f
Mixtures
Butanoic acid:isobutanoic acid:4-methylphenol:3-methylindole	18	1 μg:100 ng:10 ng:10 ng	+25^{^***}^,a,b	–3^e,f
Butanoic acid:isobutanoic acid:4-methylphenol:3-methylindole	11	1 μg:10 ng:10 ng:10 ng	+3^c	–5^e,f
Butanoic acid:isobutanoic acid:4-methylphenol:3-methylindole	20	10 ng:10 ng:10 ng:10 ng	+2^c	+6^e
^²Methane	13	70 ppm	+1^c	–6^e,f

Treatment	Ticks tested (N)	Source dose	% Attraction^¹	% Change in speed^¹
Entrained rumen fluid odour	17	1/10 dilution	+42^{^***}^,a	+4^e,f
Single components
Butanoic acid	16	10 ng	+34^{^***}^,a,b	–34^{^**}^,f,g
Isobutanoic acid	29	10 ng–1 μg	+32^{^***}^,a,b	–9^{^*}^,e,f
4-Methylphenol	11	10 ng	+24^{^**}^,b,c	–2^e
3-Methylindole	20	10 ng	+12^{^*}^,b,c	–7^e,f
Acetic acid	14	1 μg	+15^{^*}^,b,c	+3^e
Propanoic acid	20	10 ng	+22^{^***}^,a,b	–12^{^***}^,f
Mixtures
Butanoic acid:isobutanoic acid:4-methylphenol:3-methylindole	18	1 μg:100 ng:10 ng:10 ng	+25^{^***}^,a,b	–3^e,f
Butanoic acid:isobutanoic acid:4-methylphenol:3-methylindole	11	1 μg:10 ng:10 ng:10 ng	+3^c	–5^e,f
Butanoic acid:isobutanoic acid:4-methylphenol:3-methylindole	20	10 ng:10 ng:10 ng:10 ng	+2^c	+6^e
^²Methane	13	70 ppm	+1^c	–6^e,f

Median % change compared with control

P<0.05

P<0.01

P<0.001; Wilcoxon signed rank test. Treatments sharing the same letter are not significantly different; (P>0.05;Wilcoxon–Mann–Whitney test

Estimated concentration in the air stream