Primer sets (5′ to 3′) for RT-PCR
| Gene . | Forward . | Reverse . | Product (bp) . | Reference . |
|---|---|---|---|---|
| Xl_gsc | ACAACTGGAAGCACTGGA | TCTTATTCCAGAGGAACC | 278 | Xenbase* |
| Xl_cer | GCTGAACTATTTGATTCCACC | ATGGCTTGTATTCTGTGGGGC | 255 | Bouwmeester et al., 1996 |
| Xl_chd | CCTCCAATCCAAGACTCCAGCAG | GGAGGAGGAGGAGCTTTGGGACAAG | 267 | Bouwmeester et al., 1996 |
| Xl_otx2 | GGATGGATTTGTTGCACCAGTC | CACTCTCCGAGCTCACTTCTC | 315 | Sasai et al., 1995 |
| Xl_papc | CACCTGGACAATTGTGC | ATGGTTCGGTATGTGCAGG | 357 | Medina et al., 2004 |
| Xl_h4 | CGGGATAACATTCAGGGTATCACT | ATCCATGGCGGTAACTGTCTTCCT | 188 | Niehrs et al., 1994 |
| Nv_lhx1 (Fig. 1) | GCCATAGATGAGCCAAAGGA | TCCGTAGTGGCTAGGTGGAG | 414 | This study |
| Nv_lhx1 (Fig. 6) | TCCAATGTAGCGACTGCAAG | GGCTCATCTATGGCATCATCAA | 313 | This study |
| Nv_actin | ACGGGATCGTCACTAACTGG | AGGAAGGAAGGCTGGAACAT | 583 | This study |
| Hp_lhx1 | GTCCTTTAAGAGGCCCGTTT | ATGGTCCATAGGATGGTTGC | 239 | This study |
| Hp_lhx3 | ACCGGTGACGAATTCTTC | GTCGTTCTGAAATGTGATTCC | 492 | This study |
| MitCO1 | AGGCACAGCTATGAGTGT | TCATCCAGTCCCTGCTC | 292 | Yamazaki et al., 2005 |
| Nv_ef1α | ACAAGCGAACCATCGAGAAG | AACTTCCACAGGGCAATGTC | 136 | This study (qRT-PCR) |
| Nv_chd | TAGACTGCCCAAAGCCATTC | GTGCGTTGTTGGGATATGTG | 135 | This study (qRT-PCR) |
| Nv_otxA | GATGAATTCCCACGCTATGG | CGTAGGGTGAGAGCATGAAG | 85 | This study (qRT-PCR) |
| Nv_wnt2 | GACTCCAGAGAGGTGGAAAAAG | CCAACGACATGTTGGTCTTG | 94 | This study (qRT-PCR) |
| Nv_FGFa1 | TAGTGCGAGGAACATTGGAC | TGCACCGTCTCTCTTCATTG | 128 | This study (qRT-PCR) |
| Gene . | Forward . | Reverse . | Product (bp) . | Reference . |
|---|---|---|---|---|
| Xl_gsc | ACAACTGGAAGCACTGGA | TCTTATTCCAGAGGAACC | 278 | Xenbase* |
| Xl_cer | GCTGAACTATTTGATTCCACC | ATGGCTTGTATTCTGTGGGGC | 255 | Bouwmeester et al., 1996 |
| Xl_chd | CCTCCAATCCAAGACTCCAGCAG | GGAGGAGGAGGAGCTTTGGGACAAG | 267 | Bouwmeester et al., 1996 |
| Xl_otx2 | GGATGGATTTGTTGCACCAGTC | CACTCTCCGAGCTCACTTCTC | 315 | Sasai et al., 1995 |
| Xl_papc | CACCTGGACAATTGTGC | ATGGTTCGGTATGTGCAGG | 357 | Medina et al., 2004 |
| Xl_h4 | CGGGATAACATTCAGGGTATCACT | ATCCATGGCGGTAACTGTCTTCCT | 188 | Niehrs et al., 1994 |
| Nv_lhx1 (Fig. 1) | GCCATAGATGAGCCAAAGGA | TCCGTAGTGGCTAGGTGGAG | 414 | This study |
| Nv_lhx1 (Fig. 6) | TCCAATGTAGCGACTGCAAG | GGCTCATCTATGGCATCATCAA | 313 | This study |
| Nv_actin | ACGGGATCGTCACTAACTGG | AGGAAGGAAGGCTGGAACAT | 583 | This study |
| Hp_lhx1 | GTCCTTTAAGAGGCCCGTTT | ATGGTCCATAGGATGGTTGC | 239 | This study |
| Hp_lhx3 | ACCGGTGACGAATTCTTC | GTCGTTCTGAAATGTGATTCC | 492 | This study |
| MitCO1 | AGGCACAGCTATGAGTGT | TCATCCAGTCCCTGCTC | 292 | Yamazaki et al., 2005 |
| Nv_ef1α | ACAAGCGAACCATCGAGAAG | AACTTCCACAGGGCAATGTC | 136 | This study (qRT-PCR) |
| Nv_chd | TAGACTGCCCAAAGCCATTC | GTGCGTTGTTGGGATATGTG | 135 | This study (qRT-PCR) |
| Nv_otxA | GATGAATTCCCACGCTATGG | CGTAGGGTGAGAGCATGAAG | 85 | This study (qRT-PCR) |
| Nv_wnt2 | GACTCCAGAGAGGTGGAAAAAG | CCAACGACATGTTGGTCTTG | 94 | This study (qRT-PCR) |
| Nv_FGFa1 | TAGTGCGAGGAACATTGGAC | TGCACCGTCTCTCTTCATTG | 128 | This study (qRT-PCR) |