Sequence of measurements of V̇O2 during voluntary exercise on wheels, forced exercise on a motorized treadmill, and acute cold exposure in a He-O2 atmosphere (heliox)
| Days 1–4 . | Day 5 . | Day 6 . | Day 8 . | Day 9 . | Day 10 . | Day 11 . | |
|---|---|---|---|---|---|---|---|
| Access to wheel | V̇O2 recording in wheel (48 h) | Treadmill 1 | Treadmill 2 | Heliox 1 | Heliox 2 | ||
| Days 1–4 . | Day 5 . | Day 6 . | Day 8 . | Day 9 . | Day 10 . | Day 11 . | |
|---|---|---|---|---|---|---|---|
| Access to wheel | V̇O2 recording in wheel (48 h) | Treadmill 1 | Treadmill 2 | Heliox 1 | Heliox 2 | ||
Mice were weighed on days 1 (when first placed into wheels), 5 (when moved into wheel metabolic chambers), 6 (when removed from wheel chambers), and before each treadmill and heliox trial.