Proportion of Ngn2-positive cells out of the total surface mitoses or non-surface mitoses
| . | Surface division . | Non-surface division . |
|---|---|---|
| Ngn2-positive/p-vim-positive | 4% | 32% |
| Ngn2-positive/Ki67 (+++) | 5% | 44% |
| . | Surface division . | Non-surface division . |
|---|---|---|
| Ngn2-positive/p-vim-positive | 4% | 32% |
| Ngn2-positive/Ki67 (+++) | 5% | 44% |
Sections collected from six embryos at E13 or E14 were examined using a confocal microscope (40× or 100× objective).
Total number of p-vim-positive cells counted: 406 cells for S division, 234 cells for NS division.
Total number of Ki67+++ cells counted: 205 cells for S division, 143 cells for NS division.
Differences between the S and NS groups were significant(P<0.0001, chisquare test) in both detection methods.