Proportion of Ngn2-positive cells out of the total surface mitoses or non-surface mitoses
Sections collected from six embryos at E13 or E14 were examined using a confocal microscope (40× or 100× objective).
Total number of p-vim-positive cells counted: 406 cells for S division, 234 cells for NS division.
Total number of Ki67+++ cells counted: 205 cells for S division, 143 cells for NS division.
Differences between the S and NS groups were significant(P<0.0001, chisquare test) in both detection methods.
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