Development to E7 of aggregation chimaeras (2n embryo + 4n GFP-expressing embryo)
| . | . | Number of embryos recovered . | . | . | . | Number of normal embryos with epiblast from 2n embryo only (%) . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Number of implantation sites (%) . | Total (% implanted) . | GFP 4n embryo . | Non-GFP 2n embryo . | Chimaeras . | With GFP cells in Exe . | ±GFP cells in VE . | ||||
| Controls | 36 (46) | 24 (67) | 1 | 11 | 12 | 4 (100) | 11 (92) | ||||
| NT | 82 (42) | 42 (51) | 13 | 8 | 21 | 10 (91) | 7 (64) | ||||
| . | . | Number of embryos recovered . | . | . | . | Number of normal embryos with epiblast from 2n embryo only (%) . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Number of implantation sites (%) . | Total (% implanted) . | GFP 4n embryo . | Non-GFP 2n embryo . | Chimaeras . | With GFP cells in Exe . | ±GFP cells in VE . | ||||
| Controls | 36 (46) | 24 (67) | 1 | 11 | 12 | 4 (100) | 11 (92) | ||||
| NT | 82 (42) | 42 (51) | 13 | 8 | 21 | 10 (91) | 7 (64) | ||||
Exe, extra-embryonic ectoderm.
VE, visceral endoderm.