Primers used for initial amplification of rainbow trout ECaC fragment,real time PCR, 3′ and 5′ RACE and cloning of the full length coding region
| Name . | Direction . | Sequence 5′→3′ . |
|---|---|---|
| PCR of initial fragment | ||
| 260902-ECaC1F | FWD | GCCCTGGTTATTCTGCTACTG |
| 260902-ECaC2R | REV | CAAACTGGGAGAACAGAGTGAT |
| Real-time PCR | ||
| ECaC-QPCR1 | FWD | GGACCCTTCCATGTCATTCTTATT |
| ECaC-QPCR2 | REV | ACAGCCATGACAACTGTTTCC |
| β-actin | FWD | CCAACAGATGTGGATCAGCAA |
| β-actin | REV | GGTGGCACAGAGCTGAAG GGTA |
| 5′RACE | ||
| ECaC-5RACE-RT | REV | CCACCAGGAACGCATAGGCAATAA |
| ECaC-5GSP1 | REV | CAGGGCCGTTTGTCCAAAGTAATGCT |
| ECaC-5GSP2 | REV | ATCTGGGACCTCCAGTAGCAGAATA |
| 3′RACE | ||
| ECaC-3GSP1 | FWD | CCTCCCTGTGGGTGGTGTATATGA |
| ECaC-3GSP2 | FWD | CCCCATCACTCTGTTCTCCCAGTT |
| Full-length coding | ||
| ECaC-full1 | FWD | GCTTGACGTTGCATGTTCCCTTAGT |
| ECaC-full2 | REV | CTACATTTCAACCCAGTCTTGCCCAT |
| Name . | Direction . | Sequence 5′→3′ . |
|---|---|---|
| PCR of initial fragment | ||
| 260902-ECaC1F | FWD | GCCCTGGTTATTCTGCTACTG |
| 260902-ECaC2R | REV | CAAACTGGGAGAACAGAGTGAT |
| Real-time PCR | ||
| ECaC-QPCR1 | FWD | GGACCCTTCCATGTCATTCTTATT |
| ECaC-QPCR2 | REV | ACAGCCATGACAACTGTTTCC |
| β-actin | FWD | CCAACAGATGTGGATCAGCAA |
| β-actin | REV | GGTGGCACAGAGCTGAAG GGTA |
| 5′RACE | ||
| ECaC-5RACE-RT | REV | CCACCAGGAACGCATAGGCAATAA |
| ECaC-5GSP1 | REV | CAGGGCCGTTTGTCCAAAGTAATGCT |
| ECaC-5GSP2 | REV | ATCTGGGACCTCCAGTAGCAGAATA |
| 3′RACE | ||
| ECaC-3GSP1 | FWD | CCTCCCTGTGGGTGGTGTATATGA |
| ECaC-3GSP2 | FWD | CCCCATCACTCTGTTCTCCCAGTT |
| Full-length coding | ||
| ECaC-full1 | FWD | GCTTGACGTTGCATGTTCCCTTAGT |
| ECaC-full2 | REV | CTACATTTCAACCCAGTCTTGCCCAT |
FWD, forward; REV, reverse.