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Table 2.

Force depression by moderate alkalization (pH 7.4) or cAMP after cross-bridge inhibition by orthovanadate or blebbistatin during maximal Ca2+ activation

Force depression (as a % of maximum) (N)
Influencing factorOrthovanadate (10 mmol l−1)Blebbistatin (5 μmol l−1)
pH 7.4 17.5±2.2 (3) 24.0±2.9 (2) 
cAMP* 22.2±1.6 (3) 27.3 (1) 
Force depression (as a % of maximum) (N)
Influencing factorOrthovanadate (10 mmol l−1)Blebbistatin (5 μmol l−1)
pH 7.4 17.5±2.2 (3) 24.0±2.9 (2) 
cAMP* 22.2±1.6 (3) 27.3 (1) 

The skinned anterior byssus retractor muscle (ABRM) fibre bundles were maximally Ca2+ activated at pH 6.7 and force was subsequently depressed by 10 mmol l−1 orthovanadate or 5 μmol l−1 blebbistatin. A change to pH 7.4 or addition of cAMP resulted in a further depression of force(Fig. 2A). The amount of this depression is given as a percentage (mean ± s.d.) of the values before application of the inhibitors.

*

100 μmol l−1 cAMP and 2 μmol l−1cyclosporine A.

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