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Table 1.

Force depression by the cross-bridge inhibitors orthovanadate or blebbistatin depending on pH and cAMP

Force depression (as a % of maximum) (N)
Experimental backgroundOrthovanadate (10 mmol 1−1)Blebbistatin (5 μmol 1−1)
pH 6.7 71.5±5.7 (15) 51.3±5.5 (7) 
pH 7.4 90.2±2.7 (7) 79.1±2.8 (4) 
pH 6.7 + cAMP* 91.2±2.6 (4) 81.3±2.3 (3) 
Force depression (as a % of maximum) (N)
Experimental backgroundOrthovanadate (10 mmol 1−1)Blebbistatin (5 μmol 1−1)
pH 6.7 71.5±5.7 (15) 51.3±5.5 (7) 
pH 7.4 90.2±2.7 (7) 79.1±2.8 (4) 
pH 6.7 + cAMP* 91.2±2.6 (4) 81.3±2.3 (3) 

The skinned anterior byssus retractor muscle (ABRM) fibre bundles were maximally Ca2+ activated at indicated pH, and orthovanadate(Vi) or blebbistatin were applied, resulting in a force depression(Fig. 2A, Fig. 3A). The depression was measured when the force decay reached a plateau after Viapplication or 7-10 min after blebbistatin application. The values (means± s.d.) represent the amount of force depression given as a percentage of the maximal value before cross-bridge inhibition.

*

100 μmol 1−1 cAMP and 2 μmol 1−1cyclosporine A.

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