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Table 2.

The influence of substrate interactions on glycogen synthesis and lactate clearance in muscle slices from exercised fish

Substrate(s)Average amount of glycogen synthesized (μmol g-1)Average amount of lactate cleared (μmol g-1)
None, saline only 13.1±2.8 
Lactate (5 mmol l-12.4±1.1 10±3.2 
Glycerol (1 mmol l-12.8±0.8 10.7±2.3 
Palmitate (0.31 mmol l-12.7±1.1 10.9±2.8 
Lactate (5 mmol l-1) + glycerol (1 mmol l-15.2±1.8 8.6±1.8 
Glycerol (1 mmol l-1) + palmitate (0.31 mmol l-14.14±0.8 9.4±2.9 
Lactate (5 mmol l-1) + palmitate (0.31 mmol l-19.8±1.9 
Lactate (5 mmol l-1) + glycerol (1 mmol l-1) + palmitate(0.31 mmol l-19.2±2.1 
Substrate(s)Average amount of glycogen synthesized (μmol g-1)Average amount of lactate cleared (μmol g-1)
None, saline only 13.1±2.8 
Lactate (5 mmol l-12.4±1.1 10±3.2 
Glycerol (1 mmol l-12.8±0.8 10.7±2.3 
Palmitate (0.31 mmol l-12.7±1.1 10.9±2.8 
Lactate (5 mmol l-1) + glycerol (1 mmol l-15.2±1.8 8.6±1.8 
Glycerol (1 mmol l-1) + palmitate (0.31 mmol l-14.14±0.8 9.4±2.9 
Lactate (5 mmol l-1) + palmitate (0.31 mmol l-19.8±1.9 
Lactate (5 mmol l-1) + glycerol (1 mmol l-1) + palmitate(0.31 mmol l-19.2±2.1 

Muscle slices were incubated for 1 h in the concentration of substrate indicated. Average glycogen synthesized was calculated as the difference between the average at Time 0 (immediately after exercise) level and that at the end of 1 h for only those treatments in which the glycogen content at the end of 1 h was significantly different from that at Time 0 (N=8 for each treatment).

Values are means ± 1 s.e.m.

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