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Table 1.

Specific activities used to calculate extracellular lactate, glycerol or palmitate incorporation into tissue glycogen and/or CO2

 [14C]lactate
 
  
  d.p.m. g-1 tissue
 
 
Incubation conditions
 
d.p.m. μmol-1 saline
 
Glycogen
 
CO2
 
Lactate (5 mmol l-1277 686±4820 1024±275 56 450±6430 
Lactate (5 mmol l-1) + glycerol (1 mmol l-1277 686±4820 2020±326 94 830±14 689 
Lactate (5 mmol l-1) + palmitate (0.31 mmol l-1277 686±4820 1102±467 85 502±16 773 
Lactate (5 mmol l-1) + palmitate (0.31 mmol l-1) + (1 mmol l-1) glycerol 277 686±4820 730±105 101 048±20 324 
Lactate (1 mmol l-11 266 649±90 317 2629±396 16 451±2,497 
Lactate (1 mmol l-1) + glycerol (1 mmol l-1)
 
1 266 649±90 317
 
4284±288
 
29 783±3566
 
 [14C]glycerol
 
  
Glycerol (1 mmol l-1284 685±3293 285±51 859±170 
Glycerol (1 mmol l-1) + lactate (5 mmol l-1)
 
284 685±3293
 
307±66
 
1857±340
 
 [14C]palmitate
 
  
Palmitate (0.31 mmol l-1) + glycerol (1 mmol l-13 518 422±1582 107 419±13 336 
Palmitate (0.31 mmol l-1) + lactate (5 mmol l-13 518 422±1582 123 808±8761 
Palmitate (0.31 mmol l-1) + lactate (5 mmol l-1) + (1 mmol l-1) glycerol 3 518 422±1582 148 195±9547 
Palmitate (1 mmol l-11 261 840±36 627 870±236 
Palmitate (1 mmol l-1) + glycerol (1 mmol l-11 261 840±36 627 4201±648 
 [14C]lactate
 
  
  d.p.m. g-1 tissue
 
 
Incubation conditions
 
d.p.m. μmol-1 saline
 
Glycogen
 
CO2
 
Lactate (5 mmol l-1277 686±4820 1024±275 56 450±6430 
Lactate (5 mmol l-1) + glycerol (1 mmol l-1277 686±4820 2020±326 94 830±14 689 
Lactate (5 mmol l-1) + palmitate (0.31 mmol l-1277 686±4820 1102±467 85 502±16 773 
Lactate (5 mmol l-1) + palmitate (0.31 mmol l-1) + (1 mmol l-1) glycerol 277 686±4820 730±105 101 048±20 324 
Lactate (1 mmol l-11 266 649±90 317 2629±396 16 451±2,497 
Lactate (1 mmol l-1) + glycerol (1 mmol l-1)
 
1 266 649±90 317
 
4284±288
 
29 783±3566
 
 [14C]glycerol
 
  
Glycerol (1 mmol l-1284 685±3293 285±51 859±170 
Glycerol (1 mmol l-1) + lactate (5 mmol l-1)
 
284 685±3293
 
307±66
 
1857±340
 
 [14C]palmitate
 
  
Palmitate (0.31 mmol l-1) + glycerol (1 mmol l-13 518 422±1582 107 419±13 336 
Palmitate (0.31 mmol l-1) + lactate (5 mmol l-13 518 422±1582 123 808±8761 
Palmitate (0.31 mmol l-1) + lactate (5 mmol l-1) + (1 mmol l-1) glycerol 3 518 422±1582 148 195±9547 
Palmitate (1 mmol l-11 261 840±36 627 870±236 
Palmitate (1 mmol l-1) + glycerol (1 mmol l-11 261 840±36 627 4201±648 

Values are means ± 1 s.e.m. (N=8).

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