Primers used in this study
| Primer . | Forward (5′ to 3′) . | Reverse (5′ to 3′) . |
|---|---|---|
| qPCR . | . | . |
| Ck19 | CCCACGTGTTCCACAAACATC | CCATGGGAACAGTTATTTGGAGA |
| Hnf4a | TGCCTGCCTCAAAGCCAT | CACTCAGCCCCTTGGCAT |
| Oc1 | CGGAGTTCCAGCGCATGT | TCCTTCCCGTGTTCTTGCTC |
| Oc2 | CAGCATGCAAACGCAAAGA | TCTTCTGCGAGTTGTTCCTGTCT |
| Hnf1b | CCCAGCAATCTCAGAACCTC | AGGCTGCTAGCCACACTGTT |
| Hhex | GAAGACTGAAACAGGAGAATCCTCA | TCCAAACTGTCCAACGCATC |
| Sox9 | ACTCTGGGCAAGCTCTGGAG | CGAAGGGTCTCTTCTCGCTCT |
| Jag1 | CCCACGTGTTCCACAAACATC | CCATGGGAACAGTTATTTGGAGA |
| Jag2 | TCCTCCTGCTGCTTTGTGATC | TCAGGCAGGTCCCTTGCA |
| Dll1 | GATGGATCTCTGCGGCTCTTC | GCACCGGCACAGGTAAGAGT |
| Dll4 | AGCAACCCGTGTGCCAAT | TCGGCTTGGACCTCTGTTCT |
| Notch1 | CAATGTTCGAGGACCAGATGG | ACTGCAGGAGGCAATCATGAG |
| Notch2 | GACGTGCTGGACGTGAATGT | CAGGTCTGAGCTGCCTCCTC |
| Notch3 | GCACTTGCCGTGGTTACATG | CCTCACAACTGTCACCAGCATAG |
| Notch4 | CTGTGTGCCTCAGCCCAGT | TGAGCAGTTCTGTCCCTCATAGC |
| Hes1 | AAAGCCTATCATGGAGAAGAGGCG | GGAATGCCGGGAGCTATCTTTCTT |
| Hes5 | GAGATGCTCAGTCCCAAGGAGA | CGGTCCCGACGCATCTT |
| Hey1 | ACACTGCAGGAGGGAAAGGTT | CAAACTCCGATAGTCCATAGCCA |
| Hey2 | AAGCGCCCTTGTGAGGAAAC | GGTAGTTGTCGGTGAATTGGAC |
| Heyl | TCCGACGGCGAGTCTGAT | AGCGGCCTGGCCATCT |
| ChIP | ||
| Sox9 #3 | GGCTTTCTCACACGTGGGAA | TCGGAGACGGGATGTGGA |
| Sox9 #8 | GAGCATCTACAGTGCTTTTCCCA | GGAAGTGCTTGTCTACAGGGAATT |
| Sox9 #9 | GGTTCACACGGAGACCGTTC | TGCTCTCTACTCTCGGAATGTCAC |
| Sox9 #10 | GCTGCTCGGAACTGCCTG | AACTGGTAAAGTTGTCGCTCCC |
| Ctrl | TCCTGCCTTCTTTATTACTCTTAAAGC | GCAGAGGCCCTGAGACAACA |
| Primer . | Forward (5′ to 3′) . | Reverse (5′ to 3′) . |
|---|---|---|
| qPCR . | . | . |
| Ck19 | CCCACGTGTTCCACAAACATC | CCATGGGAACAGTTATTTGGAGA |
| Hnf4a | TGCCTGCCTCAAAGCCAT | CACTCAGCCCCTTGGCAT |
| Oc1 | CGGAGTTCCAGCGCATGT | TCCTTCCCGTGTTCTTGCTC |
| Oc2 | CAGCATGCAAACGCAAAGA | TCTTCTGCGAGTTGTTCCTGTCT |
| Hnf1b | CCCAGCAATCTCAGAACCTC | AGGCTGCTAGCCACACTGTT |
| Hhex | GAAGACTGAAACAGGAGAATCCTCA | TCCAAACTGTCCAACGCATC |
| Sox9 | ACTCTGGGCAAGCTCTGGAG | CGAAGGGTCTCTTCTCGCTCT |
| Jag1 | CCCACGTGTTCCACAAACATC | CCATGGGAACAGTTATTTGGAGA |
| Jag2 | TCCTCCTGCTGCTTTGTGATC | TCAGGCAGGTCCCTTGCA |
| Dll1 | GATGGATCTCTGCGGCTCTTC | GCACCGGCACAGGTAAGAGT |
| Dll4 | AGCAACCCGTGTGCCAAT | TCGGCTTGGACCTCTGTTCT |
| Notch1 | CAATGTTCGAGGACCAGATGG | ACTGCAGGAGGCAATCATGAG |
| Notch2 | GACGTGCTGGACGTGAATGT | CAGGTCTGAGCTGCCTCCTC |
| Notch3 | GCACTTGCCGTGGTTACATG | CCTCACAACTGTCACCAGCATAG |
| Notch4 | CTGTGTGCCTCAGCCCAGT | TGAGCAGTTCTGTCCCTCATAGC |
| Hes1 | AAAGCCTATCATGGAGAAGAGGCG | GGAATGCCGGGAGCTATCTTTCTT |
| Hes5 | GAGATGCTCAGTCCCAAGGAGA | CGGTCCCGACGCATCTT |
| Hey1 | ACACTGCAGGAGGGAAAGGTT | CAAACTCCGATAGTCCATAGCCA |
| Hey2 | AAGCGCCCTTGTGAGGAAAC | GGTAGTTGTCGGTGAATTGGAC |
| Heyl | TCCGACGGCGAGTCTGAT | AGCGGCCTGGCCATCT |
| ChIP | ||
| Sox9 #3 | GGCTTTCTCACACGTGGGAA | TCGGAGACGGGATGTGGA |
| Sox9 #8 | GAGCATCTACAGTGCTTTTCCCA | GGAAGTGCTTGTCTACAGGGAATT |
| Sox9 #9 | GGTTCACACGGAGACCGTTC | TGCTCTCTACTCTCGGAATGTCAC |
| Sox9 #10 | GCTGCTCGGAACTGCCTG | AACTGGTAAAGTTGTCGCTCCC |
| Ctrl | TCCTGCCTTCTTTATTACTCTTAAAGC | GCAGAGGCCCTGAGACAACA |