The Company of Biologists

Table 1.

Phenotypes of sea mutants, morphants and mRNA-rescued embryos

			Tail phenotypes (%)
Genotype	Age (dpf)	n	Straight	CTD	Abnormal	None	Cysts
sea^fa20r siblings (+/+, +/–)	2.5	96	100	–	–	100	–
sea^fa20r –/–	2.5	36	–	100	–	31	69
sea^fa20r –/–	3.5	36	–	100	–	–	100
Wild type + e5i5 sea MO	1.5	140	14	84	2	n/a	n/a
Wild type + e5i5 sea MO	2.5	127	45	55	–	29	71
Wild type + e5i5 sea MO	3.5	127	78	22	–	48	52
sea^fa20r clutch	1.5	78	73	27	–	n/a	n/a
sea^fa20r clutch + mRNA	1.5	118	100	–	–	n/a	n/a
sea^fa20r clutch	2.5	78	73	27	–	73	27
sea^fa20r clutch + mRNA	2.5	118	98	–	2	92	8
sea^tg238a clutch	1.5	292	74	26	–	n/a	n/a
sea^tg238a clutch + mRNA	1.5	52	94	6	–	n/a	n/a
sea^tg238a clutch	2.5	25	76	24	–	80	20
sea^tg238a clutch + mRNA	2.5	45	96	4	–	96	4

			Tail phenotypes (%)
Genotype	Age (dpf)	n	Straight	CTD	Abnormal	None	Cysts
sea^fa20r siblings (+/+, +/–)	2.5	96	100	–	–	100	–
sea^fa20r –/–	2.5	36	–	100	–	31	69
sea^fa20r –/–	3.5	36	–	100	–	–	100
Wild type + e5i5 sea MO	1.5	140	14	84	2	n/a	n/a
Wild type + e5i5 sea MO	2.5	127	45	55	–	29	71
Wild type + e5i5 sea MO	3.5	127	78	22	–	48	52
sea^fa20r clutch	1.5	78	73	27	–	n/a	n/a
sea^fa20r clutch + mRNA	1.5	118	100	–	–	n/a	n/a
sea^fa20r clutch	2.5	78	73	27	–	73	27
sea^fa20r clutch + mRNA	2.5	118	98	–	2	92	8
sea^tg238a clutch	1.5	292	74	26	–	n/a	n/a
sea^tg238a clutch + mRNA	1.5	52	94	6	–	n/a	n/a
sea^tg238a clutch	2.5	25	76	24	–	80	20
sea^tg238a clutch + mRNA	2.5	45	96	4	–	96	4

dpf, days post fertilization; CTD, curly tail down

Abnormal refers to a small percentage of embryos that showed various developmental defects upon injection of the e5i5 MO. These defects were not observed in uninjected controls. The same MO embryos analyzed at 2.5 dpf were analyzed at 3.5 dpf. The 2% abnormal embryos seen in the fa20r clutch injected with mRNA refer to a few embryos that had a curly tail up phenotype. In clutches of embryos, we expect 25% to be mutants

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