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Table 3.

The chronic effects of two intraperitoneal phenylhydrazine injections(at weeks 0 and 2) on the haematocrit, relative ventricular mass, percentage compact myocardium and splenic mass in cold-acclimated rainbow trout

WeekTreatmentHaematocrit (%)Relative ventricular mass (%)Percentage compact myocardium (%)Relative splenic mass (%)
Pre-treatment 38.6±1.1 0.087±0.003 29.4±1.8 0.108±0.01 
Untreated 38.6±1.9 0.095±0.003 31.1±1.4 0.156±0.01* 
 Sham-injected 36.0±2.1 0.103±0.005* 31.2±1.2 0.159±0.03* 
 PHZ-treated 15.6±1.5*† 0.118±0.004*† 37.0±1.5*+ 0.199±0.01*+ 
Untreated 34.1±1.0 0.088±0.003 29.3±1.4 0.110±0.01 
 Sham-injected 34.9±1.3 0.092±0.003 30.3±1.8 0.068±0.006 
 PHZ-treated 28.1±1.5*† 0.113±0.005*† 34.2±1.4 0.210±0.04*+ 
WeekTreatmentHaematocrit (%)Relative ventricular mass (%)Percentage compact myocardium (%)Relative splenic mass (%)
Pre-treatment 38.6±1.1 0.087±0.003 29.4±1.8 0.108±0.01 
Untreated 38.6±1.9 0.095±0.003 31.1±1.4 0.156±0.01* 
 Sham-injected 36.0±2.1 0.103±0.005* 31.2±1.2 0.159±0.03* 
 PHZ-treated 15.6±1.5*† 0.118±0.004*† 37.0±1.5*+ 0.199±0.01*+ 
Untreated 34.1±1.0 0.088±0.003 29.3±1.4 0.110±0.01 
 Sham-injected 34.9±1.3 0.092±0.003 30.3±1.8 0.068±0.006 
 PHZ-treated 28.1±1.5*† 0.113±0.005*† 34.2±1.4 0.210±0.04*+ 

Values are means ± s.e.m. (N=10 for each sample date and treatment). *Significant difference compared with the pre-treatment value, as determined by ANOVA and Dunnett's test (P<0.05); significant difference between PHZ-injected and time-matched control groups

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