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Table 2.

The chronic effects of two intraperitoneal phenylhydrazine injections(at weeks 0 and 2) on the haematocrit, relative ventricular mass, percentage compact myocardium and splenic mass in warm-acclimated rainbow trout

WeekTreatmentHaematocrit (%)Relative ventricular massCompact composition (%)Relative spleen mass
Pre-treatment 34.7±1.5 0.086±0.006 32.2±2.3 NA 
Sham-injected 36.3±2.1 0.0816±0.005 36.1±1.3 0.116±0.012 
 PHZ-injected 24.1±1.0*+ 0.126±0.008*+ 40.5±1.3*+ 0.191±0.012+ 
Sham-injected 35.6±1.5 0.105±0.003+ 33.1±2.0 0.104±0.009 
 PHZ-injected 29.3±0.7*+ 0.122±0.005*+ 35.9±1.4 0.188±0.015+ 
Sham-injected 37.9±0.9 0.097±0.003 34.6±1.6 0.100±0.110 
 PHZ-injected 23.6±1.0*+ 0.146±0.011*+ 34.1±1.2 0.173±0.007+ 
Sham-injected 32.7±1.8 0.094±0.002 34.6±1.2 0.108±0.011 
 PHZ-injected 25.8±2.1*+ 0.158±0.006*+ 37.3±1.7 0.262±0.013+ 
12 Sham-injected 30.6±1.6 0.115±0.007* 30.8±1.8 0.134±0.015 
 PHZ-injected 28.1±1.7 0.141±0.005*+ 32.0±1.2 0.214±0.060+ 
WeekTreatmentHaematocrit (%)Relative ventricular massCompact composition (%)Relative spleen mass
Pre-treatment 34.7±1.5 0.086±0.006 32.2±2.3 NA 
Sham-injected 36.3±2.1 0.0816±0.005 36.1±1.3 0.116±0.012 
 PHZ-injected 24.1±1.0*+ 0.126±0.008*+ 40.5±1.3*+ 0.191±0.012+ 
Sham-injected 35.6±1.5 0.105±0.003+ 33.1±2.0 0.104±0.009 
 PHZ-injected 29.3±0.7*+ 0.122±0.005*+ 35.9±1.4 0.188±0.015+ 
Sham-injected 37.9±0.9 0.097±0.003 34.6±1.6 0.100±0.110 
 PHZ-injected 23.6±1.0*+ 0.146±0.011*+ 34.1±1.2 0.173±0.007+ 
Sham-injected 32.7±1.8 0.094±0.002 34.6±1.2 0.108±0.011 
 PHZ-injected 25.8±2.1*+ 0.158±0.006*+ 37.3±1.7 0.262±0.013+ 
12 Sham-injected 30.6±1.6 0.115±0.007* 30.8±1.8 0.134±0.015 
 PHZ-injected 28.1±1.7 0.141±0.005*+ 32.0±1.2 0.214±0.060+ 

N=12 for each sample date and treatment, except PHZ-injected Week 12 (N=6). NA, not available

*

Significant difference compared with the pre-treatment value (mean ±s.e.m.), as determined by ANOVA and Dunnett's test (P<0.05)

+

significant difference between PHZ-injected and time-matched control groups

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