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Table 1.

Acute effects of a single intraperitoneal phenylhydrazine injection on cardiorespiratory variables in cold-acclimated rainbow trout

Haematocrit (%)Haemoglobin (g dl–1)Cardiac output (ml min–1 kg–1)Heart rate (min–1)Stroke volume (ml kg–1)
Normocythemia 22.7±1.4a 6.6±0.7a 18.3±3.2a 51.0±3.0 0.37±0.06 
Anaemic 9.4±0.6b 4.0±0.5b 21.7±3.5a 47.9±4.2 0.45±0.06 
Severely anaemic 3.6±0.5c 1.1±0.2c 33.3±3.9b 53.2±4.2 0.62±0.06 
Haematocrit (%)Haemoglobin (g dl–1)Cardiac output (ml min–1 kg–1)Heart rate (min–1)Stroke volume (ml kg–1)
Normocythemia 22.7±1.4a 6.6±0.7a 18.3±3.2a 51.0±3.0 0.37±0.06 
Anaemic 9.4±0.6b 4.0±0.5b 21.7±3.5a 47.9±4.2 0.45±0.06 
Severely anaemic 3.6±0.5c 1.1±0.2c 33.3±3.9b 53.2±4.2 0.62±0.06 

As there was some variability in the time for the onset of anaemia among fishes, sampling interval varied for each fish such that the anaemic and severe anaemic data were generally collected 24–72 h and 72–96 h,respectively, after injection. The maximal period for data collection post-injection for any fish was 4 days

Values are means ± s.e.m. (N=5). Significant differences among data for normocythemic, anaemic and severely anaemic statesare indicated by different letters, as determined by repeated-measures ANOVA and Tukey–Kramer multiple-range test (P<0.05)

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