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Table 1.

Primers used for real-time PCR

PrimerSequence (5′ to 3′)Tm °CAmplicon (bp)
Mm Zeb2 LP TAGCCGGTCCAGAAGAAATG 60.0 156 
Mm Zeb2 RP GGCCATCTCTTTCCTCCAGT 61.0  
Mm Snai1 LP AAGATGCACATCCGAAGC 57.0 199 
Mm Snai1 RP ATCTCTTCACATCCGAGTGG 58.0  
Mm Snai2 LP TGATGCCCAGTCTAGGAAAT 57.0 200 
Mm Snai2 RP AGTGAGGGCAAGAGAAAGG 57.0  
Mm Vim LP CGGCTGCGAGAGAAATTGC 57.1 124 
Mm Vim RP CCACTTTCCGTTCAAGGTCAAG 56.2  
Mm PAI1 LP TTCGGAGTAAAAGTGTTTCAGCA 54.8 176 
Mm PAI1 RP TGAGCTGTGCCCTTCTCATTG 57.5  
Mm TBX2 LP ACCAACAACATTTCTGACAAGCA 55.6 134 
Mm TBX2 RP GGGAAGACATAGGTGCGGAAG 57.6  
Mm TBX3 LP TGTCTCGAAAACCCTTTGCA 54.8 111 
Mm TBX3 RP GAACCTACCTGTTCCCGGAAA 56.8  
Mm Ets1 LP CCCAGAATCCTGTTACACCTCG 57.2 236 
Mm Ets1 RP GCTTGATGGCAAAGTAGTCTGT 55.3  
Mm ARF LP TGAGGCTAGAGAGGATCTTGAGA 56.3 91 
Mm ARF RP GCAGAAGAGCTGCTACGTGAA 57.3  
Mm p16 LP CCCAACGCCCCGAACT 58.6 79 
Mm p16 RP GCAGAAGAGCTGCTACGTGAA 57.3  
Mm p21 LP GTGGCCTTGTCGCTGTCTT 58.1 126 
Mm p21 RP GCGCTTGGAGTGATAGAAATCTG 55.9  
Mm Bmi1 LP ATCCCCACTTAATGTGTGTCCT 55.9 116 
Mm Bmi1 RP CTTGCTGGTCTCCAAGTAACG 55.7  
Mm p15 LP CCCTGCCACCCTTACCAGA 59.3 169 
Mm p15 RP CAGATACCTCGCAATGTCACG 55.7  
Mm Cdh1 LP CAGGTCTCCTCATGGCTTTGC 58.3 175 
Mm Cdh1 RP CTTCCGAAAAGAAGGCTGTCC 56.0  
Mm GAPDH LP AACGACCCCTTCATTGAC 56.0 191 
Mm GAPDH RP TCCACGACATACTCAGCAC 56.0  
Mm ACTB LP GGCTGTATTCCCCTCCATCG 57.6 154 
Mm ACTB RP CCAGTTGGTAACAATGCCATGT 55.9  
PrimerSequence (5′ to 3′)Tm °CAmplicon (bp)
Mm Zeb2 LP TAGCCGGTCCAGAAGAAATG 60.0 156 
Mm Zeb2 RP GGCCATCTCTTTCCTCCAGT 61.0  
Mm Snai1 LP AAGATGCACATCCGAAGC 57.0 199 
Mm Snai1 RP ATCTCTTCACATCCGAGTGG 58.0  
Mm Snai2 LP TGATGCCCAGTCTAGGAAAT 57.0 200 
Mm Snai2 RP AGTGAGGGCAAGAGAAAGG 57.0  
Mm Vim LP CGGCTGCGAGAGAAATTGC 57.1 124 
Mm Vim RP CCACTTTCCGTTCAAGGTCAAG 56.2  
Mm PAI1 LP TTCGGAGTAAAAGTGTTTCAGCA 54.8 176 
Mm PAI1 RP TGAGCTGTGCCCTTCTCATTG 57.5  
Mm TBX2 LP ACCAACAACATTTCTGACAAGCA 55.6 134 
Mm TBX2 RP GGGAAGACATAGGTGCGGAAG 57.6  
Mm TBX3 LP TGTCTCGAAAACCCTTTGCA 54.8 111 
Mm TBX3 RP GAACCTACCTGTTCCCGGAAA 56.8  
Mm Ets1 LP CCCAGAATCCTGTTACACCTCG 57.2 236 
Mm Ets1 RP GCTTGATGGCAAAGTAGTCTGT 55.3  
Mm ARF LP TGAGGCTAGAGAGGATCTTGAGA 56.3 91 
Mm ARF RP GCAGAAGAGCTGCTACGTGAA 57.3  
Mm p16 LP CCCAACGCCCCGAACT 58.6 79 
Mm p16 RP GCAGAAGAGCTGCTACGTGAA 57.3  
Mm p21 LP GTGGCCTTGTCGCTGTCTT 58.1 126 
Mm p21 RP GCGCTTGGAGTGATAGAAATCTG 55.9  
Mm Bmi1 LP ATCCCCACTTAATGTGTGTCCT 55.9 116 
Mm Bmi1 RP CTTGCTGGTCTCCAAGTAACG 55.7  
Mm p15 LP CCCTGCCACCCTTACCAGA 59.3 169 
Mm p15 RP CAGATACCTCGCAATGTCACG 55.7  
Mm Cdh1 LP CAGGTCTCCTCATGGCTTTGC 58.3 175 
Mm Cdh1 RP CTTCCGAAAAGAAGGCTGTCC 56.0  
Mm GAPDH LP AACGACCCCTTCATTGAC 56.0 191 
Mm GAPDH RP TCCACGACATACTCAGCAC 56.0  
Mm ACTB LP GGCTGTATTCCCCTCCATCG 57.6 154 
Mm ACTB RP CCAGTTGGTAACAATGCCATGT 55.9  
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