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Table 1.

Limb bud mesenchymal proliferation and cell death

E10
E10.5
WTFgfr2Msx2-CreFgfr1/2Prx1-CreWTFgfr2Msx2-CreFgfr1/2Prx1-Cre
pHH3
 

 

 

 

 

 

 
Forelimb 7.1 (21) 6.8 (15) 6.9 (19) 7.7 (20) 5.8 (24) 8.4 (13) 
s.d. 2.7 2.3 2.1 2.0 2.0 2.5 
P value  NS NS  0.003 NS 
Hindlimb 6.0 (16) 4.6 (18)  6.8 (5) 4.0 (5)  
s.d. 1.3 1.8  1.3 1.2  
P value
 

 
0.011
 

 

 
0.008
 

 
Caspase 3
 

 

 

 

 

 

 
Forelimb −/+ −/+ ++ −/+ −/+ 
Hindlimb −/+ −/+  −/+ +++  
E10
E10.5
WTFgfr2Msx2-CreFgfr1/2Prx1-CreWTFgfr2Msx2-CreFgfr1/2Prx1-Cre
pHH3
 

 

 

 

 

 

 
Forelimb 7.1 (21) 6.8 (15) 6.9 (19) 7.7 (20) 5.8 (24) 8.4 (13) 
s.d. 2.7 2.3 2.1 2.0 2.0 2.5 
P value  NS NS  0.003 NS 
Hindlimb 6.0 (16) 4.6 (18)  6.8 (5) 4.0 (5)  
s.d. 1.3 1.8  1.3 1.2  
P value
 

 
0.011
 

 

 
0.008
 

 
Caspase 3
 

 

 

 

 

 

 
Forelimb −/+ −/+ ++ −/+ −/+ 
Hindlimb −/+ −/+  −/+ +++  

Shown are the average number of phosphohistone H3 (pHH3)-labeled cells in a 1.5×104 μm2 area adjacent to the AER, with the number of sections examined from at least three embryos in parentheses. Low frequency of caspase 3 (or TUNEL, not shown)-positive cells (−/+);transient proximal cell death (++); slightly increased distal mesenchymal cell death (+); cell death throughout the entire limb bud mesenchyme (+++).

NS, Not significantly different from wild type (WT).

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