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Table 3.

Left-right Cai2+ levels in chick embryos treated with serotonin agonists and antagonists, or proton ATPase antagonists

Mean Fura-2 signal from line-scan analysis
TreatmentRightLeftRatio of left/right Cai2+ enrichment
Untreated 171.1±9.4 (3) 186.6±12.2 2.55±0.21 (3) 
Fluoxetine, 10 μM 143.5±23.0 (11)* 158.3±17.7** 1.38±0.13 (6)** 
2-Methyl-5HT, 25 μM 135.0±21.8 (11)** 153.6±17.6** 0.52±0.26 (4)** 
Ondansetron, 25 μM 188.4±19.2 (10)* 200.5±8.7* 0.97±0.25 (6)** 
ML 10302, 25 μM 119.8±18.8 (9)** 130.2±25.5** 0.82±0.08 (6)** 
GR 125487, 25 μM 188.3±10.8 (10)** 195.4±10.9 1.03±0.05 (10)** 
Concanamycin, 100 nM 151.2±13.7 (13)** 150.8±13.6** 1.04±0.24 (6)** 
Lansoprozole, 7 μM 186.1±13.7 (14)** 183.8±18.1 1.03±0.19 (7)** 
Mean Fura-2 signal from line-scan analysis
TreatmentRightLeftRatio of left/right Cai2+ enrichment
Untreated 171.1±9.4 (3) 186.6±12.2 2.55±0.21 (3) 
Fluoxetine, 10 μM 143.5±23.0 (11)* 158.3±17.7** 1.38±0.13 (6)** 
2-Methyl-5HT, 25 μM 135.0±21.8 (11)** 153.6±17.6** 0.52±0.26 (4)** 
Ondansetron, 25 μM 188.4±19.2 (10)* 200.5±8.7* 0.97±0.25 (6)** 
ML 10302, 25 μM 119.8±18.8 (9)** 130.2±25.5** 0.82±0.08 (6)** 
GR 125487, 25 μM 188.3±10.8 (10)** 195.4±10.9 1.03±0.05 (10)** 
Concanamycin, 100 nM 151.2±13.7 (13)** 150.8±13.6** 1.04±0.24 (6)** 
Lansoprozole, 7 μM 186.1±13.7 (14)** 183.8±18.1 1.03±0.19 (7)** 

Left and right mean Fura-2 signal, relative Cai2+levels and the LR Cai2+ ratio were calculated as described in the Materials and methods. The number of embryos analyzed is indicated in parentheses. Asterisks indicate that values differ from HH6 controls on that side at *P<0.05 and **P<0.005 using an unpaired t-test.

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