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Table 3.

glp-1 expression in AWC neurons is sufficient to correct glp-1-dependent dauer maintenance defects

Transgene injected*Rescue of dauer maintenance defect±s.d.
myo-2p::GFP 6.1±5.4% 
gpa-2p::glp-1 70.0±9.6% 
odr-1p::glp-1 76.5±7.5% 
ser-2prom2::glp-1 14.3±3.8% 
lim-6int3::glp-1 5.0±4.5% 
unc-25p::glp-1 15.5±6.2% 
ceh-6p::glp-1 6.6±12.6% 
Transgene injected*Rescue of dauer maintenance defect±s.d.
myo-2p::GFP 6.1±5.4% 
gpa-2p::glp-1 70.0±9.6% 
odr-1p::glp-1 76.5±7.5% 
ser-2prom2::glp-1 14.3±3.8% 
lim-6int3::glp-1 5.0±4.5% 
unc-25p::glp-1 15.5±6.2% 
ceh-6p::glp-1 6.6±12.6% 

Neuronal promoters were used to drive GLP-1 expression in various types of neurons to determine whether neuron-specific GLP-1 expression would suppress the dauer maintenance defects associated with glp-1 If mutations. p, promoter.

*

All constructs were injected with myo-2p::GFP as a co-transformation marker in the parental strain glp-1(e2141)daf-7(e1372).

See Materials and methods; s.d., standard deviation; n=100.

Statistical analyses were performed: P<0.005, compared with control myo-2::GFP-expressing transgenic lines.

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