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Table 3.

BioPlex Protein Array analysis of COC-secreted cytokine and chemokine family members (pg/ml) secreted following stimulation with LPS, Pam3Cys or HA fragments

ControlLPSPam3CysHyaluronan fragment
IL1α 6.34±1.51 47.93±5.33 60.9±5.07 7.02±5.69 
IL1β 35.01±8.15 78.04±6.66 15.3±2.54 
IL6 9.16±2.33 344.84±28.62 472.18±52.63 284.35±18.72 
IL9 72.23±30.51 65.11±6.25 
IL12 (p40) 0.08±0.02 36.14±6.94 28.69±3.99 33.33±5.11 
IL12 (p70) 52.4±10.36 139.18±11.56 196.32±21.68 27.87±6.33 
GCSF 17.61±5.65 173.96±35.63 265.59±32.83 26.71±3.75 
MCP1 23.51±3.89 468.82±55.88 506.21±42.68 255.82±20.63 
MIP1α 5.13±1.17 182.78±30.42 404.74±25.88 2.85±0.06 
MIP1β 11.07±2.20 1082.73±105.36 927.3±62.16 678.56±72.39 
RANTES 24.19±5.26 1368.25±256.82 474.58±48.67 674.24±51.78 
ControlLPSPam3CysHyaluronan fragment
IL1α 6.34±1.51 47.93±5.33 60.9±5.07 7.02±5.69 
IL1β 35.01±8.15 78.04±6.66 15.3±2.54 
IL6 9.16±2.33 344.84±28.62 472.18±52.63 284.35±18.72 
IL9 72.23±30.51 65.11±6.25 
IL12 (p40) 0.08±0.02 36.14±6.94 28.69±3.99 33.33±5.11 
IL12 (p70) 52.4±10.36 139.18±11.56 196.32±21.68 27.87±6.33 
GCSF 17.61±5.65 173.96±35.63 265.59±32.83 26.71±3.75 
MCP1 23.51±3.89 468.82±55.88 506.21±42.68 255.82±20.63 
MIP1α 5.13±1.17 182.78±30.42 404.74±25.88 2.85±0.06 
MIP1β 11.07±2.20 1082.73±105.36 927.3±62.16 678.56±72.39 
RANTES 24.19±5.26 1368.25±256.82 474.58±48.67 674.24±51.78 

Ovulated COCs (50 COCs per/well) were cultured with 100 ng/ml LPS, 1μg/ml Pam3Cys or 100 μg/ml of HA fragments for 24 hours. Media samples were collected and cytokines present in the media were analyzed using a Bio-Plex Mouse Cytokine 23-Plex Panel.

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