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Table 2.

List of antibodies used for western blotting

AntibodyCompanyDilution
β-Actin (AC74) Sigma 1:10,000 
CCR3 Anaspec, San Hose, CA 1:250 
ERK 1/2 (p44/p42 MAP kinase, 9102) Cell Signaling, Boston, MA 1:1000 
pERK 1/2 (phospho-p44/p42 MAPK, E10, 9106) Cell Signaling 1:2000 
IκB-a (L35A5, 4814) Cell Signaling 1:2000 
IRF3 Cell Signaling 1:1000 
MYD88 eBioscience, San Diago, CA 1:1000 
NF-κB (3034) Cell Signaling 1:1000 
phospho-NF-κB p65 (Ser536, 3031) Cell Signaling 1:1000 
phospho-p38MAPK (28B10, 9126) Cell Signaling 1:2000 
Toll-like receptor 2 (2229) Cell Signaling 1:1000 
Toll-like receptor 4 (IMG578A) IMGENEX, San Diago, CA 1:500 
phospho-Tyr (P-Tyr-100, 9411) Cell Signaling 1:5000 
AntibodyCompanyDilution
β-Actin (AC74) Sigma 1:10,000 
CCR3 Anaspec, San Hose, CA 1:250 
ERK 1/2 (p44/p42 MAP kinase, 9102) Cell Signaling, Boston, MA 1:1000 
pERK 1/2 (phospho-p44/p42 MAPK, E10, 9106) Cell Signaling 1:2000 
IκB-a (L35A5, 4814) Cell Signaling 1:2000 
IRF3 Cell Signaling 1:1000 
MYD88 eBioscience, San Diago, CA 1:1000 
NF-κB (3034) Cell Signaling 1:1000 
phospho-NF-κB p65 (Ser536, 3031) Cell Signaling 1:1000 
phospho-p38MAPK (28B10, 9126) Cell Signaling 1:2000 
Toll-like receptor 2 (2229) Cell Signaling 1:1000 
Toll-like receptor 4 (IMG578A) IMGENEX, San Diago, CA 1:500 
phospho-Tyr (P-Tyr-100, 9411) Cell Signaling 1:5000 
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