Measured dynamics from a temporal ICCM analysis of α5-integrin and α-actinin from regions of a CHO B2 cell
| Region . | Integrin/α-actinin complex D×10-11 (cm2 s-1) . | Integrin/α-actinin complex |v| (μm min-1) . |
|---|---|---|
| 1 | 1.4±0.2 | - |
| 2* | 1.7±0.2 | 0.50±0.10 |
| 3* | 1.3±0.1 | 0.40±0.07 |
| 4* | 1.6±0.3 | 0.64±0.05 |
| 5 | 4.0±0.4 | - |
| 6* | 2.0±0.2 | 0.59±0.07 |
| 7 | 1.9±0.1 | - |
| 8* | 1.0±0.1 | 0.60±0.03 |
| 9† | 0.8±0.4 | 1.01±0.07 |
| 10* | 1.0±0.2 | 0.4±0.1 |
| Region . | Integrin/α-actinin complex D×10-11 (cm2 s-1) . | Integrin/α-actinin complex |v| (μm min-1) . |
|---|---|---|
| 1 | 1.4±0.2 | - |
| 2* | 1.7±0.2 | 0.50±0.10 |
| 3* | 1.3±0.1 | 0.40±0.07 |
| 4* | 1.6±0.3 | 0.64±0.05 |
| 5 | 4.0±0.4 | - |
| 6* | 2.0±0.2 | 0.59±0.07 |
| 7 | 1.9±0.1 | - |
| 8* | 1.0±0.1 | 0.60±0.03 |
| 9† | 0.8±0.4 | 1.01±0.07 |
| 10* | 1.0±0.2 | 0.4±0.1 |
One protein population that is both diffusing and flowing. †Two protein populations, one that is diffusing and one that is flowing. See Fig. 8A-F. Data were fit with one of Equations 11-13 and the fit with the best R2 value was chosen.