Table 4.

Measured temporal ICM parameters for α-actinin on regions of a CHO B2 cell expressing α-actinin-GFP near a disassembling adhesion

First 70 frames
Last 30 frames
Region D×10-11* (cm2 s-1) |v|* (μm min-1) Immobile* (%) D×10-11 (cm2 s-1) Immobile (%)
1   6±2   0.25±0.06   28   2.6±0.8   64  
2   1.7±0.5   0.10±0.03   13   0.13±0.04   0  
3   3.1±0.9   0.29±0.01   0   —   50  
4   1.8±0.5   0.18±0.02   0   0.21±0.06   0  
5   0.8±0.2   0.15±0.03   15   —   50  
6   1.1±0.3   0.16±0.05   41   0.6±0.2   0  
7   0.6±0.2   0.11±0.01   6   0.6±0.2   6  
First 70 frames
Last 30 frames
Region D×10-11* (cm2 s-1) |v|* (μm min-1) Immobile* (%) D×10-11 (cm2 s-1) Immobile (%)
1   6±2   0.25±0.06   28   2.6±0.8   64  
2   1.7±0.5   0.10±0.03   13   0.13±0.04   0  
3   3.1±0.9   0.29±0.01   0   —   50  
4   1.8±0.5   0.18±0.02   0   0.21±0.06   0  
5   0.8±0.2   0.15±0.03   15   —   50  
6   1.1±0.3   0.16±0.05   41   0.6±0.2   0  
7   0.6±0.2   0.11±0.01   6   0.6±0.2   6  
*

,values for first 70 frames of movie. , values for last 30 frames of movie. See also Fig. 5C,D. Diffusion coefficients, flow speeds and percentage of immobile protein are given for the given regions of interest.

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