Measured temporal ICM parameters for α-actinin on regions of a CHO B2 cell expressing α-actinin-GFP near a disassembling adhesion
. | First 70 frames . | . | . | Last 30 frames . | . | |||
---|---|---|---|---|---|---|---|---|
Region . | D×10-11* (cm2 s-1) . | |v|* (μm min-1) . | Immobile* (%) . | D×10-11† (cm2 s-1) . | Immobile† (%) . | |||
1 | 6±2 | 0.25±0.06 | 28 | 2.6±0.8 | 64 | |||
2 | 1.7±0.5 | 0.10±0.03 | 13 | 0.13±0.04 | 0 | |||
3 | 3.1±0.9 | 0.29±0.01 | 0 | — | 50 | |||
4 | 1.8±0.5 | 0.18±0.02 | 0 | 0.21±0.06 | 0 | |||
5 | 0.8±0.2 | 0.15±0.03 | 15 | — | 50 | |||
6 | 1.1±0.3 | 0.16±0.05 | 41 | 0.6±0.2 | 0 | |||
7 | 0.6±0.2 | 0.11±0.01 | 6 | 0.6±0.2 | 6 |
. | First 70 frames . | . | . | Last 30 frames . | . | |||
---|---|---|---|---|---|---|---|---|
Region . | D×10-11* (cm2 s-1) . | |v|* (μm min-1) . | Immobile* (%) . | D×10-11† (cm2 s-1) . | Immobile† (%) . | |||
1 | 6±2 | 0.25±0.06 | 28 | 2.6±0.8 | 64 | |||
2 | 1.7±0.5 | 0.10±0.03 | 13 | 0.13±0.04 | 0 | |||
3 | 3.1±0.9 | 0.29±0.01 | 0 | — | 50 | |||
4 | 1.8±0.5 | 0.18±0.02 | 0 | 0.21±0.06 | 0 | |||
5 | 0.8±0.2 | 0.15±0.03 | 15 | — | 50 | |||
6 | 1.1±0.3 | 0.16±0.05 | 41 | 0.6±0.2 | 0 | |||
7 | 0.6±0.2 | 0.11±0.01 | 6 | 0.6±0.2 | 6 |
,values for first 70 frames of movie. †, values for last 30 frames of movie. See also Fig. 5C,D. Diffusion coefficients, flow speeds and percentage of immobile protein are given for the given regions of interest.