Measured temporal and spatial ICM parameters for α-actinin on regions of a CHO B2 cell
| Region . | D×10-11 (cm2 s-1) . | <N>diffusing (μm-2) . | |v| (μm min-1) . | <N>flowing (μm-2) . | <N>immobile (μm-2) . | <DA> . |
|---|---|---|---|---|---|---|
| 3 | 0.78±0.08 | 6.9±0.7 | — | — | — | 1.7 |
| 5 | 1.8±0.8 | 8±3 | 0.23±0.08 | 3±1 | 3±2 | 1.7 |
| 6 | 1.88±0.08 | 2.1±0.7 | — | — | 0.82±0.03 | 6.2 |
| 7 | 2.1±0.2 | 4.2±0.2 | — | — | 3.3±0.2 | 2.2 |
| 8 | 0.4±0.1 | 0.8±0.2 | — | — | 0.4±0.2 | 7.8 |
| 9 | 0.94±0.05 | 0.33±0.01 | — | — | 1.35±0.03 | 5.4 |
| 10 | <0.1 | — | — | — | — | — |
| 11 | 1.9±0.1 | 0.34±0.01 | — | — | 1.05±0.03 | 5.0 |
| 12 | 1.8±0.2 | 2.3±0.1 | - | - | 3.2±0.2 | 1.9 |
| 13 | 0.38±0.02 | 4.8±0.2 | - | - | 3.4±0.2 | 5.3 |
| 14 | 1.23±0.09 | 9.9±0.5 | — | — | 4.2±0.3 | 1.4 |
| 15 | 3±1 | 9±2 | 0.25±0.1 | 5±1 | 6±2 | 0.9 |
| 16 | 2±1 | 2±1 | 0.20±0.04 | 1.4±1.2 | 1±1 | 1.6 |
| Region . | D×10-11 (cm2 s-1) . | <N>diffusing (μm-2) . | |v| (μm min-1) . | <N>flowing (μm-2) . | <N>immobile (μm-2) . | <DA> . |
|---|---|---|---|---|---|---|
| 3 | 0.78±0.08 | 6.9±0.7 | — | — | — | 1.7 |
| 5 | 1.8±0.8 | 8±3 | 0.23±0.08 | 3±1 | 3±2 | 1.7 |
| 6 | 1.88±0.08 | 2.1±0.7 | — | — | 0.82±0.03 | 6.2 |
| 7 | 2.1±0.2 | 4.2±0.2 | — | — | 3.3±0.2 | 2.2 |
| 8 | 0.4±0.1 | 0.8±0.2 | — | — | 0.4±0.2 | 7.8 |
| 9 | 0.94±0.05 | 0.33±0.01 | — | — | 1.35±0.03 | 5.4 |
| 10 | <0.1 | — | — | — | — | — |
| 11 | 1.9±0.1 | 0.34±0.01 | — | — | 1.05±0.03 | 5.0 |
| 12 | 1.8±0.2 | 2.3±0.1 | - | - | 3.2±0.2 | 1.9 |
| 13 | 0.38±0.02 | 4.8±0.2 | - | - | 3.4±0.2 | 5.3 |
| 14 | 1.23±0.09 | 9.9±0.5 | — | — | 4.2±0.3 | 1.4 |
| 15 | 3±1 | 9±2 | 0.25±0.1 | 5±1 | 6±2 | 0.9 |
| 16 | 2±1 | 2±1 | 0.20±0.04 | 1.4±1.2 | 1±1 | 1.6 |
See also Fig. 5B. Diffusion coefficients, flow speeds, and number densities per unit area squared are given for diffusing, flowing, and immobile integrin populations in given regions of interest. The DA for the first image in the time series is also shown. Note that these DA values are calculated from two photon images and do not correspond to the GAP-GFP normalized values.