Measured temporal and spatial ICM parameters for α5-integrin on image regions of a CHO B2 cell expressing α5-integrin-GFP
| Region . | D×10-11 (cm2s-1) . | |v| (μm min-1) . | Diffusing (%) . | Flowing (%) . | Immobile (%) . | <DA> . |
|---|---|---|---|---|---|---|
| 1 | 2.4±0.4 | 0.7±0.1 | 53±5 | 7±2 | 39±3 | 7 |
| 2 | 0.14±0.02 | - | - | - | 100±6* | 6 |
| 3 | 0.5±0.2 | - | 30±5 | - | 70±8 | 3 |
| 4 | 0.11±0.02 | 1.7±0.3 | - | 4±1 | 96±10* | 6 |
| 5 | 0.04±0.02 | 0.9±0.2 | - | 3±1 | 97±59* | 6 |
| 6 | - | 0.58±0.04 | - | 20±1 | 80±1 | 6 |
| 7 | 3±1 | - | 13±1 | - | 87±2 | 6 |
| 8 | 0.7±0.4 | - | 23±6 | - | 77±9 | 7 |
| Region . | D×10-11 (cm2s-1) . | |v| (μm min-1) . | Diffusing (%) . | Flowing (%) . | Immobile (%) . | <DA> . |
|---|---|---|---|---|---|---|
| 1 | 2.4±0.4 | 0.7±0.1 | 53±5 | 7±2 | 39±3 | 7 |
| 2 | 0.14±0.02 | - | - | - | 100±6* | 6 |
| 3 | 0.5±0.2 | - | 30±5 | - | 70±8 | 3 |
| 4 | 0.11±0.02 | 1.7±0.3 | - | 4±1 | 96±10* | 6 |
| 5 | 0.04±0.02 | 0.9±0.2 | - | 3±1 | 97±59* | 6 |
| 6 | - | 0.58±0.04 | - | 20±1 | 80±1 | 6 |
| 7 | 3±1 | - | 13±1 | - | 87±2 | 6 |
| 8 | 0.7±0.4 | - | 23±6 | - | 77±9 | 7 |
These areas show diffusion but the rate is so slow it is considered essentially immobile. See also Fig. 5A. Diffusion coefficients, flow speeds, and percentage of protein within each protein population are given for diffusing, flowing, and immobile integrin populations in given regions of interest. The DA normalized versus GAP-GFP for the first image in the time series is also shown.