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Table 2.

Efficiency of tyrosine phosphorylation of different CEACAM1-L variants after pervanadate treatment

CEACAM1-L variant Tyr-P: CEACAM1 Localization
Wild-type   1   L  
Δ518   0.50   L  
S503A   14.9   AP  
V518A   0.46   L  
3K-3A   272.0   AP  
CEACAM1-L variant Tyr-P: CEACAM1 Localization
Wild-type   1   L  
Δ518   0.50   L  
S503A   14.9   AP  
V518A   0.46   L  
3K-3A   272.0   AP  

Partially polarized cells were treated with pervanadate and solubilized in 1% Triton X-100. CEACAM1-L was immunoprecipitated and the ratio of Tyr phosphorylation levels to CEACAM1 protein levels (Tyr-P: CEACAM1) was determined by western blot with anti-phosphorylated-Tyr antibodies and anti-CEACAM1 antibodies, and quantified in a Fuji Film detector system.

AP, exclusively apical localization; L, lateral plus apical localization;

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