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Table 1.

Normal and mutant Dictyostelium strains

NameParentGenotype (reference)
NC-4 Wild-type  
Ax3 NC-4 axeA/B/C 
Ax3-RFP Ax3 act15::RFP 
HW285 Ax3 phyA+, dscB::phyA-myc 
HW288 Ax3 phyA(=P4H1), clone 1a(van der Wel et al., 2005) 
HW289 Ax3 phyA, clone 1b(van der Wel et al., 2005) 
P4H1-RFP HW288 phyA, act15::RFP 
HW401 HW288 phyA, dscB::phyA-myc (semi-constitutive) 
HW402 HW288 phyA, phyA::phyA-myc 
HW403 HW288 phyA, cotB::phyA-myc (prespore) 
HW404 HW288 phyA, ecmA(FL)::phyA-myc(prestalk) 
HW405 HW288 phyA, pspA::lacZ (prespore) 
HW406 HW288 phyA, ecmA(construct O)::lacZ(PstA) (Early et al., 1993) 
HW407 HW288 phyA, ecmA(construct S)::lacZ(PstA) (Zhukovskaya et al.,2006) 
HW408 HW288 phyA, ecmA0::lacZ (PstA & 0)(Early et al., 1993) 
HW409 HW288 phyA, ecm0::lacZ (Pst0)(Early et al., 1993) 
HW410 HW288 phyA, pkaC::pkaC(Anjard et al., 1992) 
HW415 Ax3 phyA::RFP 
HW421 Ax3 dscB::phyA-myc (semi-constitutive) 
HW422 Ax3 phyA::phyA-myc 
HW423 Ax3 cotB::phyA-myc (prespore) 
HW424 Ax3 ecmA(FL)::phyA-myc (prestalk) 
HW425 Ax3 pspA::lacZ (prespore) 
HW426 Ax3 ecmA(construct O)::lacZ (PstA)(Early et al., 1993) 
HW427 Ax3 ecmA(construct S)::lacZ (PstA)(Zhukovskaya et al., 2006) 
HW428 Ax3 ecmA0::lacZ (PstA & 0) (Early et al., 1993) 
HW429 Ax3 ecm0::lacZ (Pst0) (Early et al.,1993) 
HW430 Ax3 pkaC::pkaC (Anjard et al.,1992) 
HW260 Ax3 pgtA (van der Wel et al., 2002a) 
HW263 HW260 pgtA, cotB::phyA 
HW264 HW260 pgtA, ecmA(FL)::phyA 
HW265 HW260 pgtA, act15::RFP 
HR6 Ax3 fbxA (Ennis et al., 2000) 
NameParentGenotype (reference)
NC-4 Wild-type  
Ax3 NC-4 axeA/B/C 
Ax3-RFP Ax3 act15::RFP 
HW285 Ax3 phyA+, dscB::phyA-myc 
HW288 Ax3 phyA(=P4H1), clone 1a(van der Wel et al., 2005) 
HW289 Ax3 phyA, clone 1b(van der Wel et al., 2005) 
P4H1-RFP HW288 phyA, act15::RFP 
HW401 HW288 phyA, dscB::phyA-myc (semi-constitutive) 
HW402 HW288 phyA, phyA::phyA-myc 
HW403 HW288 phyA, cotB::phyA-myc (prespore) 
HW404 HW288 phyA, ecmA(FL)::phyA-myc(prestalk) 
HW405 HW288 phyA, pspA::lacZ (prespore) 
HW406 HW288 phyA, ecmA(construct O)::lacZ(PstA) (Early et al., 1993) 
HW407 HW288 phyA, ecmA(construct S)::lacZ(PstA) (Zhukovskaya et al.,2006) 
HW408 HW288 phyA, ecmA0::lacZ (PstA & 0)(Early et al., 1993) 
HW409 HW288 phyA, ecm0::lacZ (Pst0)(Early et al., 1993) 
HW410 HW288 phyA, pkaC::pkaC(Anjard et al., 1992) 
HW415 Ax3 phyA::RFP 
HW421 Ax3 dscB::phyA-myc (semi-constitutive) 
HW422 Ax3 phyA::phyA-myc 
HW423 Ax3 cotB::phyA-myc (prespore) 
HW424 Ax3 ecmA(FL)::phyA-myc (prestalk) 
HW425 Ax3 pspA::lacZ (prespore) 
HW426 Ax3 ecmA(construct O)::lacZ (PstA)(Early et al., 1993) 
HW427 Ax3 ecmA(construct S)::lacZ (PstA)(Zhukovskaya et al., 2006) 
HW428 Ax3 ecmA0::lacZ (PstA & 0) (Early et al., 1993) 
HW429 Ax3 ecm0::lacZ (Pst0) (Early et al.,1993) 
HW430 Ax3 pkaC::pkaC (Anjard et al.,1992) 
HW260 Ax3 pgtA (van der Wel et al., 2002a) 
HW263 HW260 pgtA, cotB::phyA 
HW264 HW260 pgtA, ecmA(FL)::phyA 
HW265 HW260 pgtA, act15::RFP 
HR6 Ax3 fbxA (Ennis et al., 2000) 

A series analogous to HW401-404 was established in HW289 as HW411-414. Independent clones were established and analyzed for other strains. Under genotype is listed promoter::coding region.

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