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Table 3.

Loss of ESCRT-I disrupts transport of EGFR to the lysosome

Lamin-A RNAi (from 9 cells)
 
    

 
Lysosomes
 
MVBs
 
Unclassified
 
Autophagosomes
 
108 particles   1515   35   169   19  
Percentage of total (1738)   87.2   2.0   9.7   1.1  
TSG101 RNAi (from 10 cells containing MC structures)
 
    

 
Lysosomes
 
Cisternae
 
Unclassified*
 
Autophagosomes
 
108 particles   50   272   296   54  
Percentage of total (672)   7.4   40.5   44.0   8.0  
Lamin-A RNAi (from 9 cells)
 
    

 
Lysosomes
 
MVBs
 
Unclassified
 
Autophagosomes
 
108 particles   1515   35   169   19  
Percentage of total (1738)   87.2   2.0   9.7   1.1  
TSG101 RNAi (from 10 cells containing MC structures)
 
    

 
Lysosomes
 
Cisternae
 
Unclassified*
 
Autophagosomes
 
108 particles   50   272   296   54  
Percentage of total (672)   7.4   40.5   44.0   8.0  

HeLa cells were incubated with EGF and 108 anti-EGFR 10 nm gold for 30 minutes at 4°C, washed, and incubated at 37°C for a further 3 hours, with TfHRP added for the final 30 minutes. Gold particles were counted in classes of intracellular membranes; lysosomes, MVBs and autophagosomes were scored by morphology. Cisternae were scored as endosomes containing TfHRP in part of a matrix-linked structure.

*

Note that in TSG101 cells many `unclassified' structures were small TfHRP-containing structures close to multicisternae, and were probably linked to them outside the plane of the section.

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