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Table 2.

rga-2 acts in dorsal/ventral cells and can be suppressed by stronger junctions


A










Genotype
Plasmid
Line*
Lethal
n
Trans
rga-2(hd102) prga-2::rga-2::gfp (construct P13) E8 39 688 75 
rga-2(hd102) pelt-3::rga-2(cDNA) e1 67.3 453 43.6 
 pelt-3::rga-2(cDNA) e2 79 81 28.4 
 pelt-3::rga-2(cDNA) e3 71 1741 40 
 pelt-3::rga-2(cDNA) e4 62 197 51.7 
rga-2(hd102) pceh-16::rga-2(cDNA) 4/4 lines 99   
rga-2(hd102)§ pelt-3::rga-2(cDNA) (e4) + pceh-16::rga-2(cDNA) 61 249  
 pelt-3::rga-2(cDNA) (e4) + pceh-16::rga-2(cDNA) 64 182  
rga-2(hd102) prga-2::rga-2::gfp::CAAX 6/6 lines 99   
rga-2(hd102) prga-2::rga-2::gfp::SAAX 48 397  
 prga-2::rga-2::gfp::SAAX 29 292  

 
prga-2::rga-2::gfp::SAAX
 
6
 
48
 
309
 

 

A










Genotype
Plasmid
Line*
Lethal
n
Trans
rga-2(hd102) prga-2::rga-2::gfp (construct P13) E8 39 688 75 
rga-2(hd102) pelt-3::rga-2(cDNA) e1 67.3 453 43.6 
 pelt-3::rga-2(cDNA) e2 79 81 28.4 
 pelt-3::rga-2(cDNA) e3 71 1741 40 
 pelt-3::rga-2(cDNA) e4 62 197 51.7 
rga-2(hd102) pceh-16::rga-2(cDNA) 4/4 lines 99   
rga-2(hd102)§ pelt-3::rga-2(cDNA) (e4) + pceh-16::rga-2(cDNA) 61 249  
 pelt-3::rga-2(cDNA) (e4) + pceh-16::rga-2(cDNA) 64 182  
rga-2(hd102) prga-2::rga-2::gfp::CAAX 6/6 lines 99   
rga-2(hd102) prga-2::rga-2::gfp::SAAX 48 397  
 prga-2::rga-2::gfp::SAAX 29 292  

 
prga-2::rga-2::gfp::SAAX
 
6
 
48
 
309
 

 
B





Genotype
Plasmid (injection)
Line
Lethal
n
Trans
rga-2(hd102)/hln1pdlg-1::dlg-1::rfp 2 ng/μL12637523.5
pdlg-1::dlg-1::rfp 10 ng/μL12112819
pdlg-1::dlg-1::rfp 10 ng/μL219.511322.2
rga-2(hd102)pdlg-1::dlg-1::rfp 2 ng/μL198.228323.5
pdlg-1::dlg-1::rfp 10 ng/μL282.79822.2
dpy-5(e61)rga-2(hd102)None97.71262
dpy-5(e61)rga-2(hd102); mcls46
pdlg-1::dlg-1::rfp integrated
1
81.9
950

B





Genotype
Plasmid (injection)
Line
Lethal
n
Trans
rga-2(hd102)/hln1pdlg-1::dlg-1::rfp 2 ng/μL12637523.5
pdlg-1::dlg-1::rfp 10 ng/μL12112819
pdlg-1::dlg-1::rfp 10 ng/μL219.511322.2
rga-2(hd102)pdlg-1::dlg-1::rfp 2 ng/μL198.228323.5
pdlg-1::dlg-1::rfp 10 ng/μL282.79822.2
dpy-5(e61)rga-2(hd102)None97.71262
dpy-5(e61)rga-2(hd102); mcls46
pdlg-1::dlg-1::rfp integrated
1
81.9
950

*

Line number.

Percentage lethality.

Percentage of transgene transmission in the progeny of heterozygous animals.

§

The plasmid pceh-16::rga-2(cDNA) was injected with myo-2::gfp into the transgenic line e4 obtained with pelt-3::rga-2(cDNA) and pRF4.

Three additional lines failed to rescue.

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