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Table 1.

Genetic interactions between rga-2, let-502 and mel-11




Control

rga-2(RNAi) feeding

rga-2(RNAi) injection
Genotype
Temp
Let
n
Let
n
Let
n
A
 

 

 

 

 

 

 

 
20°C 263 511 35 693 
rrf-3(pk1426) 20°C 206 74 109 88 43 
mel-11(sb56)unc-4(e120)/mnC1* 20°C 128 23.5 1192   
mel-11(it26) unc-4(e120)sqt-1(sc13)/mnC1* 20°C 16 183 25 257   
let-502(sb118ts) 20°C 233 526 6.5 702 
let-502(sb118ts) 25°C 95 106 33 718   
let-502(sb118ts) 26°C 100 130 100 304 79 444 
let-502(ca201sb54)/+ 20°C 111 353 133 
nmy-1(sb115)/sZT1 20°C 35 115 43 609 36** 144 
rga-2(hd102) 20°C 100 1320     
rga-2(hd102)/hDf17§ 20°C 100 256     
let-502(sb118ts) dpy-5(e61) 20°C 714     
let-502(sb118ts) dpy-5(e61) rga-2(hd102) 20°C 32†† 858     
let-502(sb118ts) dpy-5(e61) rga-2(hd102)
 
25°C
 
99
 
340
 

 

 

 

 
B
 

 
Let
 
n
 
Let
 
n
 
Let
 
n
 
Ctrl   <1 1205 32‡‡,§§ 755 
+HS   <1 760 36‡‡,§§ 116 
nzls1[hs::rho-1(G14V)] Ctrl 411 1191 81‡‡,§§ 558 
nzls1[hs::rho-1(G14V)] +HS 417 1031 74‡‡,§§ 150 
let-502(sb118ts) Ctrl 88 392     
let-502(sb118ts)¶¶ +HS 89 357     
let-502(sb118ts) nzls1[hs::rho-1(G14V)] Ctrl 74 303     
let-502(sb118ts)nzls1[hs::rho-1(G14V)]¶¶
 
+HS
 
67
 
302
 

 

 

 

 



Control

rga-2(RNAi) feeding

rga-2(RNAi) injection
Genotype
Temp
Let
n
Let
n
Let
n
A
 

 

 

 

 

 

 

 
20°C 263 511 35 693 
rrf-3(pk1426) 20°C 206 74 109 88 43 
mel-11(sb56)unc-4(e120)/mnC1* 20°C 128 23.5 1192   
mel-11(it26) unc-4(e120)sqt-1(sc13)/mnC1* 20°C 16 183 25 257   
let-502(sb118ts) 20°C 233 526 6.5 702 
let-502(sb118ts) 25°C 95 106 33 718   
let-502(sb118ts) 26°C 100 130 100 304 79 444 
let-502(ca201sb54)/+ 20°C 111 353 133 
nmy-1(sb115)/sZT1 20°C 35 115 43 609 36** 144 
rga-2(hd102) 20°C 100 1320     
rga-2(hd102)/hDf17§ 20°C 100 256     
let-502(sb118ts) dpy-5(e61) 20°C 714     
let-502(sb118ts) dpy-5(e61) rga-2(hd102) 20°C 32†† 858     
let-502(sb118ts) dpy-5(e61) rga-2(hd102)
 
25°C
 
99
 
340
 

 

 

 

 
B
 

 
Let
 
n
 
Let
 
n
 
Let
 
n
 
Ctrl   <1 1205 32‡‡,§§ 755 
+HS   <1 760 36‡‡,§§ 116 
nzls1[hs::rho-1(G14V)] Ctrl 411 1191 81‡‡,§§ 558 
nzls1[hs::rho-1(G14V)] +HS 417 1031 74‡‡,§§ 150 
let-502(sb118ts) Ctrl 88 392     
let-502(sb118ts)¶¶ +HS 89 357     
let-502(sb118ts) nzls1[hs::rho-1(G14V)] Ctrl 74 303     
let-502(sb118ts)nzls1[hs::rho-1(G14V)]¶¶
 
+HS
 
67
 
302
 

 

 

 

 

Each experiment was performed at least two (A) or three (B) times.

+, wild type.

+HS, Heat shock.

Ctrl, Control experiment.

Let, Percentage embryonic lethality, except for experiments involving let-502(sb118) at 25°, in which case it reflects embryonic and larval lethality scored 72 hours after hatching.

n, The total number of larvae laid by heterozygous animals.

*

RNAi feeding was performed using mel-11 unc-4/mnC1 heterozygotes;lethality was calculated taking into account viable Unc progeny (presumptive mel-11 unc-4 homozygotes). For instance, mel-11(sb56)unc-4(e120)/mnC1 animals lay 7% dead eggs, 18% sterile coiler Uncs(presumptive mel-11), 25% sterile Dpy paralyzed Uncs (presumptive mnC1) and 50% wild-type animals, suggesting that dead eggs correspond to homozygous mel-11(sb56) embryos. Dead eggs had a ventral bulge. The small percentage of embryonic lethality observed in the progeny of heterozygous mel-11(sb56)/+ and mel-11(it26)/+ mothers is consistent with the original description of these alleles (Wissmann et al.,1999). We reproducibly saw the phenotype they reported for mel-11(it26) embryos laid by homozygous mel-11(it26)mothers.

let-502(ca201sb54) animals are viable but sterile; nmy-1(sb115) is semi-dominant. Lethality was calculated taking into account all the progeny of the corresponding heterozygous strains.

Inferred from the progeny of rga-2(hd102)/hln1[unc-54(h1040)]heterozygotes: 25% of animals were paralysed (presumptive hln1[unc-54] animals); 25% of embryos did not hatch and the rest were normal; thus, >99% rga-2(hd102) embryos die.

§

Inferred from the F1 cross progeny of rga-2(hd102)/hln1[unc-54(h1040)] males and hDf17/hln1[unc-54(h1040)] hermaphrodites. Dead embryos had a ventral bulge.

Percentage of larval lethality; dying larvae were as illustrated in Fig. 1E.

**

Arrested progeny had the hypoelongation phenotype of nmy-1(sb115)larvae rather than the bulge phenotype of rga-2-deficient embryos.

††

Arrested embryos had the characteristic bulge of rga-2(hd102)embryos.

‡‡

Injection in N2 and nzls1 animals was on the same day and with the same rga-2 double-stranded RNA (dsRNA) preparation. Dead embryos had a ventral bulge.

§§

Using a different rga-2 dsRNA preparation, we found 13% lethality for N2 without (n=614) and 16% after heat shock (n=253), 67%lethality after injection into the nzls1 background without(n=499) and 67% after heat shock (n=203).

¶¶

The difference between heat-shocked let-502(sb118ts) and let-502(sb118ts) nzls1[hs::rho-1(G14V)] is statistically significant(P<0.01).

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