Skip to Main Content
Table 2.

Particle speeds in different mutant backgrounds and under the experimental conditions indicated

Strain/experimentParticlesTemp.Speed¶¶n***
Wild-type young adults* DIC particles 25°C 7.8±2.4 6; 21 
 DIC particles 22°C 6.0±2.1 3; 29 
 Oil drop injection†† 25°C 3.9±1.0 3; 3 
 DIC particles after oil drop injection†† 25°C 4.2±1.1 3; 50 
 Microspheres‡‡ ND§§ 4.8±1.0 2; 13 
 PGL-1::GFP particles‡‡ ND§§ 4.3±1.1 2; 15 
Wild-type before first ovulation DIC particles 22°C 3.7±1.3 3; 33 
Extruded gonads in EGTA* DIC particles 22°C 5.0±2.7 7; 47 
Old wild-type adults DIC particles 22°C 0.0±0.3 3; 21 
 PGL-1::GFP particles ND§§ 0.2±0.2 2; 15 
Old wild-type after mating, DIC particles 22°C 5.3±1.9 5; 43 
fem-1(hc-17ts) young DIC particles 22°C 4.1±2.0 4; 38 
fem-1(hc-17ts) old§ DIC particles 22°C −0.2±0.6 3; 16 
fem-3(q20gf) DIC particles 22°C 5.2±1.5 5; 30 
fog-1(q241) XO DIC particles 22°C 2.5±0.8 4; 45 
rme-2(b1008) DIC particles 22°C 4.8±1.5 4; 30 
ced-3(n717);ced-1(e1735)** DIC particles 22°C 4.2±1.8 6; 36 
tba-2 RNAi DIC particles 20°C 7.2±1.7 6; 45 
Control RNAi DIC particles 20°C 8.1±3.0 2; 12 
Strain/experimentParticlesTemp.Speed¶¶n***
Wild-type young adults* DIC particles 25°C 7.8±2.4 6; 21 
 DIC particles 22°C 6.0±2.1 3; 29 
 Oil drop injection†† 25°C 3.9±1.0 3; 3 
 DIC particles after oil drop injection†† 25°C 4.2±1.1 3; 50 
 Microspheres‡‡ ND§§ 4.8±1.0 2; 13 
 PGL-1::GFP particles‡‡ ND§§ 4.3±1.1 2; 15 
Wild-type before first ovulation DIC particles 22°C 3.7±1.3 3; 33 
Extruded gonads in EGTA* DIC particles 22°C 5.0±2.7 7; 47 
Old wild-type adults DIC particles 22°C 0.0±0.3 3; 21 
 PGL-1::GFP particles ND§§ 0.2±0.2 2; 15 
Old wild-type after mating, DIC particles 22°C 5.3±1.9 5; 43 
fem-1(hc-17ts) young DIC particles 22°C 4.1±2.0 4; 38 
fem-1(hc-17ts) old§ DIC particles 22°C −0.2±0.6 3; 16 
fem-3(q20gf) DIC particles 22°C 5.2±1.5 5; 30 
fog-1(q241) XO DIC particles 22°C 2.5±0.8 4; 45 
rme-2(b1008) DIC particles 22°C 4.8±1.5 4; 30 
ced-3(n717);ced-1(e1735)** DIC particles 22°C 4.2±1.8 6; 36 
tba-2 RNAi DIC particles 20°C 7.2±1.7 6; 45 
Control RNAi DIC particles 20°C 8.1±3.0 2; 12 

Age of worms (days after last molt): *0.5-1 days; very young worms right at or after molt; 5 days; §2-4 days.

Speeds of two types of particles were measured in the same worms: ††after injection of a small oil drop and ‡‡after injection of fluorescent microspheres.

Worms analyzed 4.8±2.3 hours after mating to wild-type males.

**

ced-1 mutation used to confirm ced-3 phenotype.

§§

In confocal experiments, the temperature was within a range of 15-25°C.

¶¶

Average speed in μm/minute±s.d.

***

Number of worms analyzed; total number of particles analyzed.

Close Modal

or Create an Account

Close Modal
Close Modal