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Table 1.

Antibodies and labels

Primary antibodies
AntigenAntibodiesDilutionSourceSecondary antibodies (numbers correspond to secondary antibodies, below)
β3-tubulin Rabbit monoclonal 1/1000 Covance, Richmont, CA 2 or 4 (ABC kit, DAB) 
β-galactosidase Rabbit polyclonal 1/500 5′-3′, Boulder, CO 
B-FABP Rabbit polyclonal 1/500 Gift from T. Müller, Max Delbruck Center for Molecular Medicine, AG Medical Genetics, Berlin, Germany 1 or 2 
Dbh Rabbit polyclonal 1/500 Chemicon, Temecula, CA 4 (ABC kit, DAB) 
Dbh Sheep polyclonal 1/500 Chemicon 
GAP-43 Mouse monoclonal 1/5000 Gift from D. Schreyer, Stanford University, Palo Alto, CA 
HuC/D Mouse monoclonal biotinylated 1/10 Invitrogen-Molecular Probes, Carlsbad, CA (Steptavidin Alexa-Fluor-488), (Alexa-Fluor-594) or (ABC kit, DAB) 
Kit Goat polyclonal (C14) 1/1000 Santa Cruz Biotechnology, 8 (Steptavidin Alexa-Fluor-594) 
MAP2 Chicken 1/1000 Chemicon 10 
nNOS Rabbit polyclonal 1/500 Santa Cruz Biotechnology, 
p75NTR Rabbit polyclonal 1/1500 Gift from M. Chao, Skirball Institute, New York University, NY 1, 2 or 4 (Streptavidin Alexa-Fluor-350) 
PGP9.5 Rabbit polyclonal 1/1000 Biogenesis, Poole, UK 2 or 4 (ABC kit, DAB) 
Phox2b Rabbit polyclonal 1/1000 Gift from J.-F. Brunet, CNRS, École normale supérieure, Paris,France 3 or 4 (ABC kit, DAB) 
Ret Goat polyclonal 1/200 R&D Systems, Berkeley, CA 8 (ABC kit, DAB) 
Sox10 Goat polyclonal 1/10 Gift from D. Anderson, California Institute of Technology, Howard Hughes Medical Institute, Pasadena, CA 7 or 8 (ABC kit, DAB) 
Th Sheep polyclonal 1/500 Chemicon 
Primary antibodies
AntigenAntibodiesDilutionSourceSecondary antibodies (numbers correspond to secondary antibodies, below)
β3-tubulin Rabbit monoclonal 1/1000 Covance, Richmont, CA 2 or 4 (ABC kit, DAB) 
β-galactosidase Rabbit polyclonal 1/500 5′-3′, Boulder, CO 
B-FABP Rabbit polyclonal 1/500 Gift from T. Müller, Max Delbruck Center for Molecular Medicine, AG Medical Genetics, Berlin, Germany 1 or 2 
Dbh Rabbit polyclonal 1/500 Chemicon, Temecula, CA 4 (ABC kit, DAB) 
Dbh Sheep polyclonal 1/500 Chemicon 
GAP-43 Mouse monoclonal 1/5000 Gift from D. Schreyer, Stanford University, Palo Alto, CA 
HuC/D Mouse monoclonal biotinylated 1/10 Invitrogen-Molecular Probes, Carlsbad, CA (Steptavidin Alexa-Fluor-488), (Alexa-Fluor-594) or (ABC kit, DAB) 
Kit Goat polyclonal (C14) 1/1000 Santa Cruz Biotechnology, 8 (Steptavidin Alexa-Fluor-594) 
MAP2 Chicken 1/1000 Chemicon 10 
nNOS Rabbit polyclonal 1/500 Santa Cruz Biotechnology, 
p75NTR Rabbit polyclonal 1/1500 Gift from M. Chao, Skirball Institute, New York University, NY 1, 2 or 4 (Streptavidin Alexa-Fluor-350) 
PGP9.5 Rabbit polyclonal 1/1000 Biogenesis, Poole, UK 2 or 4 (ABC kit, DAB) 
Phox2b Rabbit polyclonal 1/1000 Gift from J.-F. Brunet, CNRS, École normale supérieure, Paris,France 3 or 4 (ABC kit, DAB) 
Ret Goat polyclonal 1/200 R&D Systems, Berkeley, CA 8 (ABC kit, DAB) 
Sox10 Goat polyclonal 1/10 Gift from D. Anderson, California Institute of Technology, Howard Hughes Medical Institute, Pasadena, CA 7 or 8 (ABC kit, DAB) 
Th Sheep polyclonal 1/500 Chemicon 
Secondary antibodies
Antibodies antigenAntibodiesDilutionSourceLabel
1- Rabbit Goat 1/600 Molecular probes, Eugene, OR Alexa-Fluor-488 
2- Rabbit Goat 1/600 Molecular probes Alexa-Fluor-594 
3- Rabbit Donkey 1/300 Molecular probes Alexa-Fluor-594 
4- Rabbit Donkey 1/200 Jackson ImmunoResearch, West Grove, PA Biotin 
5- Mouse Goat 1/600 Molecular probe Alexa-Fluor-488 
6- Goat Donkey 1/300 Molecular probes Alexa-Fluor-594 
7- Goat Donkey 1/300 Jackson ImmunoResearch FITC 
8- Goat Donkey 1/200 Jackson ImmunoResearch Biotin 
9- Sheep Donkey 1/300 Molecular probes Alexa-Fluor-488 
10- Chicken Goat 1/400 Molecular probes Alexa-Fluor-488 
Secondary antibodies
Antibodies antigenAntibodiesDilutionSourceLabel
1- Rabbit Goat 1/600 Molecular probes, Eugene, OR Alexa-Fluor-488 
2- Rabbit Goat 1/600 Molecular probes Alexa-Fluor-594 
3- Rabbit Donkey 1/300 Molecular probes Alexa-Fluor-594 
4- Rabbit Donkey 1/200 Jackson ImmunoResearch, West Grove, PA Biotin 
5- Mouse Goat 1/600 Molecular probe Alexa-Fluor-488 
6- Goat Donkey 1/300 Molecular probes Alexa-Fluor-594 
7- Goat Donkey 1/300 Jackson ImmunoResearch FITC 
8- Goat Donkey 1/200 Jackson ImmunoResearch Biotin 
9- Sheep Donkey 1/300 Molecular probes Alexa-Fluor-488 
10- Chicken Goat 1/400 Molecular probes Alexa-Fluor-488 
Other labels
NameDilutionSource
Streptavidin Alexa-Fluor-488, −594 or −350 1/300 Molecular probes 
Vectastain ABC kit  Vector Laboratories, UK 
DAB  Vector Laboratories 
Other labels
NameDilutionSource
Streptavidin Alexa-Fluor-488, −594 or −350 1/300 Molecular probes 
Vectastain ABC kit  Vector Laboratories, UK 
DAB  Vector Laboratories 
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