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Table 2.

Percent of embryos with developmental defects under different conditions

Genotype Moderate defects in syncytial embryos Severe defects in syncytial embryos Severe defects in cellular embryos Wild-type embryos
cbs RNAi at 18°C, n=53   5.7%   0%   0%   94.3%  
cbs RNAi at 25°C, n=98   40.8%   17.3%   7.1%   34.7%  
cbs RNAi at 29°C, n=127   13.4%   68.5%   14.2%   3.9%  
Arf72A1, n=78   (0) 0%   37.2%   62.8%   0%  
Genotype Moderate defects in syncytial embryos Severe defects in syncytial embryos Severe defects in cellular embryos Wild-type embryos
cbs RNAi at 18°C, n=53   5.7%   0%   0%   94.3%  
cbs RNAi at 25°C, n=98   40.8%   17.3%   7.1%   34.7%  
cbs RNAi at 29°C, n=127   13.4%   68.5%   14.2%   3.9%  
Arf72A1, n=78   (0) 0%   37.2%   62.8%   0%  
Centrosome hypertrophy Extra centrosomes Severe syncytial defects Wild-type embryos
GFP::GRIP at 25°C, n=27   37.0%   14.8%   7.4%   40.7%  
GFP::GRIP at 29°C, n=39   38.5%   28.2%   15.4%   17.9%  
Centrosome hypertrophy Extra centrosomes Severe syncytial defects Wild-type embryos
GFP::GRIP at 25°C, n=27   37.0%   14.8%   7.4%   40.7%  
GFP::GRIP at 29°C, n=39   38.5%   28.2%   15.4%   17.9%  

Cbs RNAi embryos were scored based on Table 1. Arf72A mutant embryos were scored based on Table 1 and overall morphology. GFP::GRIP embryos were scored based on centrosome number per nucleus and morphology, as well as overall morphology of the embryo

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