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Table 1.

Development of embryos cloned from HSCs and other somatic cells

Strain Donor cell Cultured 2 cells (%) 4 cells (%) Morulae and blastocysts (%) Embryos transferred (ET)* Implanted (% per ET) Offspring (% per ET)
BDF1 (female)   HSC   444   384 (86.5)   151 (34.0)  13/220 (5.9)§  109   24 (22.0)  0 (0.0)  
  Cumulus   1141   1029 (90.2)   746 (65.4)  88/192 (45.8)§  545   219 (40.2)  8 (1.5)  
  Fibroblasts   358   348 (97.2)   279 (77.9)  –   279   151 (54.1)  2 (0.7)  
B6×129 (male)   HSC   637   563 (88.4)   302 (47.4)  –   302   90 (29.8)**  2 (0.7)†† 
  Sertoli   175   155 (88.6)   104 (59.4)  –   81   36 (44.4)**  6 (7.4)†† 
  Fibroblasts   192   172 (89.6)   126 (65.6)  65/94 (69.1)   155   81 (52.3)**  3 (1.9)  
Strain Donor cell Cultured 2 cells (%) 4 cells (%) Morulae and blastocysts (%) Embryos transferred (ET)* Implanted (% per ET) Offspring (% per ET)
BDF1 (female)   HSC   444   384 (86.5)   151 (34.0)  13/220 (5.9)§  109   24 (22.0)  0 (0.0)  
  Cumulus   1141   1029 (90.2)   746 (65.4)  88/192 (45.8)§  545   219 (40.2)  8 (1.5)  
  Fibroblasts   358   348 (97.2)   279 (77.9)  –   279   151 (54.1)  2 (0.7)  
B6×129 (male)   HSC   637   563 (88.4)   302 (47.4)  –   302   90 (29.8)**  2 (0.7)†† 
  Sertoli   175   155 (88.6)   104 (59.4)  –   81   36 (44.4)**  6 (7.4)†† 
  Fibroblasts   192   172 (89.6)   126 (65.6)  65/94 (69.1)   155   81 (52.3)**  3 (1.9)  
*

Embryos were transferred at the 4-cell stage, except for 65 out of 155 embryos from B6×129 fibroblast clones, which were transferred at the morula or blastocyst stage

P<1×10–10

P<0.005

§

P<5×10–10

P<0.0005

**

P<0.05

††

P<0.01 (HSC vs other donor cells)

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