Percentage co-localization of mAtg9 with TGN46 upon starvation after knocking down Atg proteins
| Culture medium . | FM . | ES . | . | . | . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| RNAi . | siC . | siC . | ULK1 . | ULK2 . | Beclin . | Atg5 . | Atg7 . | Atg12 . | ||||||
| Average±s.e.m. | 51.2±1.2% | 29.1±1.7% | 61.6±1.9% | 32.9±2.6% | 41.6±1.6% | 31.9±1.9% | 32.7±1.5% | 34.4±2.2% | ||||||
| Culture medium . | FM . | ES . | . | . | . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| RNAi . | siC . | siC . | ULK1 . | ULK2 . | Beclin . | Atg5 . | Atg7 . | Atg12 . | ||||||
| Average±s.e.m. | 51.2±1.2% | 29.1±1.7% | 61.6±1.9% | 32.9±2.6% | 41.6±1.6% | 31.9±1.9% | 32.7±1.5% | 34.4±2.2% | ||||||
Images were analysed using the co-localization function of the Zeiss LSM-510 software. Quantification was performed using a region of interest surrounding the TGN staining and data are the mean values of 10 cells from a representative experiment