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Table 2.

Percentage co-localization of mAtg9 with TGN46 upon starvation after knocking down Atg proteins

Culture medium FM ES
RNAi siC siC ULK1 ULK2 Beclin Atg5 Atg7 Atg12
Average±s.e.m.   51.2±1.2%   29.1±1.7%   61.6±1.9%   32.9±2.6%   41.6±1.6%   31.9±1.9%   32.7±1.5%   34.4±2.2%  
Culture medium FM ES
RNAi siC siC ULK1 ULK2 Beclin Atg5 Atg7 Atg12
Average±s.e.m.   51.2±1.2%   29.1±1.7%   61.6±1.9%   32.9±2.6%   41.6±1.6%   31.9±1.9%   32.7±1.5%   34.4±2.2%  

Images were analysed using the co-localization function of the Zeiss LSM-510 software. Quantification was performed using a region of interest surrounding the TGN staining and data are the mean values of 10 cells from a representative experiment

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