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Table 1.

Quantification of GFP-LC3, Rab7 and mAtg9 co-localization

GFP-LC3 with Rab7 Rab7 with GFP-LC3 GFP-LC3 with mAtg9 mAtg9 with GFP-LC3 Rab7 with mAtg9 mAtg9 with Rab7
51.2±9.4%   6.7±1.2%   45.9±0.6%   7.4±0.7%   26.8±3.7%   32.3±5.0%  
GFP-LC3 with Rab7 Rab7 with GFP-LC3 GFP-LC3 with mAtg9 mAtg9 with GFP-LC3 Rab7 with mAtg9 mAtg9 with Rab7
51.2±9.4%   6.7±1.2%   45.9±0.6%   7.4±0.7%   26.8±3.7%   32.3±5.0%  

Images were analysed using the co-localization function of the Zeiss LSM-510 software. Quantification was performed for each cell, using a region of interest, and data are the mean values of 20 cells obtained from two independent experiments, %±s.e.m.

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